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  4. Fish metallothionein(MT)ELISA kit

Fish metallothionein(MT)ELISA kit

  • 中文名称：
    鱼金属硫蛋白(MT)酶联免疫试剂盒
  • 货号：
    CSB-EQ027262FI
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Fish metallothionein (MT) ELISA Kit was designed for the quantitative measurement of Fish metallothionein (MT) protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 25 pg/mL-400 pg/mL and the sensitivity is 12.5 pg/mL.

  • 缩写：
    MT
  • 种属：
    Fish
  • 样本类型：
    serum, plasma
  • 检测范围：
    25 pg/mL-400 pg/mL
  • 灵敏度：
    12.5 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Epigenetics and Nuclear Signaling
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of fish MT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:100 Average % 84  
    Range % 80-88  
    1:200 Average % 90  
    Range % 86-94  
    1:400 Average % 99  
    Range % 94-103  
    1:800 Average % 92  
    Range % 88-96  
  • 回收率：
    The recovery of fish MT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 97 92-102  
    EDTA plasma (n=4) 95 89-99  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    400 0.103 0.100 0.102    
    200 0.247 0.228 0.238    
    100 0.401 0.387 0.394    
    50 0.646 0.598 0.622    
    25 0.983 0.966 0.975    
    0 1.572 1.529 1.551    
  • 数据处理：
  • 货期：
    3-5 working days

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