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Fish growth hormone,GH ELISA Kit

  • 中文名称：
    鱼生长激素(GH)酶联免疫试剂盒
  • 货号：
    CSB-E12121Fh
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Fish growth hormone (GH) ELISA Kit was designed for the quantitative measurement of Fish growth hormone (GH) protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 312.5 pg/mL-5000 pg/mL and the sensitivity is 312.5 pg/mL.

  • 别名：
    gh1 ELISA Kit; Somatotropin-1 ELISA Kit; Growth hormone I ELISA Kit; Somatotropin I ELISA Kit
  • 缩写：
    GH
  • Uniprot No.：
  • 种属：
    Fish
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    312.5 pg/mL-5000 pg/mL
  • 灵敏度：
    312.5 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Developmental Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of fish GH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 95  
    Range % 91-98  
    1:2 Average % 92  
    Range % 88-96  
    1:4 Average % 102  
    Range % 96-106  
    1:8 Average % 96  
    Range % 92-101  
  • 回收率：
    The recovery of fish GH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 92-98  
    EDTA plasma (n=4) 94 90-98  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    5000 0.271 0.265 0.268    
    2500 0.526 0.514 0.520    
    1250 0.887 0.863 0.875    
    625 1.664 1.684 1.674    
    312.5 2.557 2.602 2.580    
  • 本试剂盒所含材料：
    • A 96-well Assay plate --The 96-well plate has been pre-coated with anti-Fish GH antibody.
    • Standard (6 x 1 ml) --Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
    • HRP-avidin (1 x 6 ml) --Catalyze the TMB to elicit a chromogenic reaction.
    • Biotin-conjugated GH (1 x 6 ml) --Bind to the precoated antibody and act as the detection antigen.
    • Sample Diluent (2 x 20 ml) --Reconstitute the standard and dilute the sample to an appropriate concentration.
    • Wash Buffer (20x concentrate) (1 x 15 ml) --Wash away unbound or free substances.
    • Substrate A (1x 7 ml) --Mix with Sbustrate B.
    • Substrate B (1x 7 ml) --Mix with Sbustrate A
    • Stop Solution (1 x 7ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells)
    • An Instruction manual

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  • 本试剂盒不含材料：
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
    • An incubator that can provide stable incubation conditions up to 37°C±5°
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water

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  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 功能：
    Growth hormone plays an important role in growth control and is involved in the regulation of several anabolic processes. Implicated as an osmoregulatory substance important for seawater adaptation.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Somatotropin/prolactin family