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  4. Fish Prolactin/Luteotropic Hormone(PRL/LTH) ELISA Kit

Fish Prolactin/Luteotropic Hormone(PRL/LTH) ELISA Kit

  • 中文名称：
    鱼催乳素(PRL/LTH)酶联免疫试剂盒
  • 货号：
    CSB-E12695Fh
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Fish PRL/LTH ELISA Kit was designed for the quantitative measurement of Fish PRL/LTH protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 10 μIU/mL-4000 μIU/mL and the sensitivity is 10 μIU/mL.

  • 别名：
    prl1 ELISA Kit; Prolactin-1 ELISA Kit; PRL-188 ELISA Kit; Prolactin I ELISA Kit
  • 缩写：
    PRL/LTH
  • Uniprot No.：
  • 种属：
    Fish
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    10 μIU/mL-4000 μIU/mL
  • 灵敏度：
    10 μIU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of fish PRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 95  
    Range % 91-100  
    1:2 Average % 95  
    Range % 86-104  
    1:4 Average % 90  
    Range % 85-95  
    1:8 Average % 96  
    Range % 92-108  
  • 回收率：
    The recovery of fish PRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 91-102  
    EDTA plasma (n=4) 95 88-105  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μIU/ml OD1 OD2 Average 　  
    4000 0.350 0.364 0.357 　  
    750 0.697 0.705 0.701 　  
    125 1.196 1.203 1.200 　  
    35 1.523 1.519 1.521 　  
    10 1.700 1.707 1.704 　  
    0 2.244 2.242 2.243 　  
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

Which fish species / family is it optimized for? We work with a non-model species and would like to check its reactivity. Thank you.

A:
Thanks for your inquiry.
CSB-E12695Fh Fish Prolactin/Luteotropic Hormone(PRL/LTH) ELISA Kit
This kit is designed against salmon. There is no requirement for fish species. Gnerally all kinds of fish can be detected out. We suggest you do a pretest.
Pls let me know if you have any further questions. Thank you.

靶点详情

  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Somatotropin/prolactin family
  • 数据库链接：

    UniGene: Oni.44217