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  4. Fish Interleukin-8,IL-8 ELISA Kit

Fish Interleukin-8,IL-8 ELISA Kit

  • 中文名称：
    鱼白介素8(IL-8/CXCL8)酶联免疫试剂盒
  • 货号：
    CSB-E12877Fh
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Fish IL8/CXCL8 ELISA Kit was designed for the quantitative measurement of Fish IL8/CXCL8 protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 31.25 pg/mL-500 pg/mL and the sensitivity is 31.25 pg/mL.

  • 缩写：
    IL8/CXCL8
  • Uniprot No.：
  • 种属：
    Fish
  • 样本类型：
    serum, plasma
  • 检测范围：
    31.25 pg/mL-500 pg/mL
  • 灵敏度：
    31.25 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<15%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of fish IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 93    
    Range % 92-97    
    1:2 Average % 95    
    Range % 85-100    
    1:4 Average % 93    
    Range % 89-101    
    1:8 Average % 95    
    Range % 86-100    
  • 回收率：
    The recovery of fish IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 96 89-100    
    EDTA plasma (n=4) 95 85-105    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    pg/ml OD1 OD2 Average 　    
    500 0.172 0.176 0.174 　    
    250 0.392 0.399 0.396 　    
    125 0.635 0.628 0.632 　    
    62.5 1.038 1.046 1.042 　    
    31.25 2.456 2.417 2.437 　    
  • 数据处理：
  • 货期：
    3-5 working days

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