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  4. Fish Insulin,INS ELISA Kit

Fish Insulin,INS ELISA Kit

  • 中文名称：
    鱼胰岛素(INS)酶联免疫试剂盒
  • 货号：
    CSB-E12123Fh
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Fish INS ELISA Kit was designed for the quantitative measurement of Fish INS protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 100 pg/mL-1600 pg/mL and the sensitivity is 100 pg/mL.

  • 缩写：
  • Uniprot No.：
  • 种属：
    Fish
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    100 pg/mL-1600 pg/mL
  • 灵敏度：
    100 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of fish INS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 86-100  
    1:2 Average % 98  
    Range % 92-103  
    1:4 Average % 85  
    Range % 80-89  
    1:8 Average % 91  
    Range % 83-99  
  • 回收率：
    The recovery of fish INS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 93 88-98  
    EDTA plasma (n=4) 92 90-95  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    1600 0.178 0.189 0.184    
    800 0.322 0.340 0.331    
    400 0.610 0.634 0.622    
    200 1.257 1.230 1.244    
    100 2.335 2.475 2.405    
  • 数据处理：
  • 货期：
    3-5 working days

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问答及客户评论

■ 常见问题解答
Q:

我想找一款可以检测斑马鱼胰岛素水平的ELISA试剂盒。你有推荐的么？

A:
我们有以下鱼试剂盒： #CSB-E12123Fh，鱼胰岛素，INS ELISA Kit 一般来说，同一物种间的蛋白质差异不大。也就是说，不同类型鱼类的氨基酸序列相对保守 这个项目#CSB-E12123Fh是专门针对鲑鱼的。我们没有测试过斑马鱼。但应该没有什么大问题，你可以试着先做一个预实验。