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Fish Heat Shock Protein 70(HSP-70)ELISA Kit

  • 中文名称：
    鱼热休克蛋白70(HSP-70)酶联免疫试剂盒
  • 货号：
    CSB-E16327Fh
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Fish HSP70 ELISA Kit was designed for the quantitative measurement of Fish HSP70 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 18.75 pg/mL-300 pg/mL and the sensitivity is 18.75 pg/mL.

  • 别名：
    Heat shock 70 kDa protein 1 ELISA Kit; HSP70-1 ELISA Kit
  • 缩写：
    HSP-70
  • Uniprot No.：
  • 种属：
    Fish
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    18.75 pg/mL-300 pg/mL
  • 灵敏度：
    18.75 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of fish HSP-70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 91  
    Range % 86-95  
    1:2 Average % 98  
    Range % 92-103  
    1:4 Average % 82  
    Range % 80-85  
    1:8 Average % 91  
    Range % 83-99  
  • 回收率：
    The recovery of fish HSP-70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 87-97  
    EDTA plasma (n=4) 94 91-98  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    300 0.153 0.144 0.149    
    150 0.255 0.242 0.249    
    75 0.519 0.533 0.526    
    37.5 1.103 1.134 1.119    
    18.75 2.363 2.270 2.317    
  • 数据处理：
  • 货期：
    3-5 working days

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