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  4. Fish Glutathione Peroxidase (GSH-PX) ELISA kit

Fish Glutathione Peroxidase (GSH-PX) ELISA kit

  • 中文名称：
    鱼谷胱甘肽过氧化物酶(GSH-PX)酶联免疫试剂盒
  • 货号：
    CSB-E15930Fh
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Fish GSH-PX ELISA Kit was designed for the quantitative measurement of Fish GSH-PX protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 375 μU/mL-6000 μU/mL and the sensitivity is 187.5 μU/mL.

  • 缩写：
    GSH-PX
  • 种属：
    Fish
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    375 μU/mL-6000 μU/mL
  • 灵敏度：
    187.5 μU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of fish GSH-PX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:100 Average % 95  
    Range % 91-99  
    1:200 Average % 103  
    Range % 99-107  
    1:400 Average % 98  
    Range % 93-103  
    1:800 Average % 92  
    Range % 87-97  
  • 回收率：
    The recovery of fish GSH-PX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 99 94-104  
    EDTA plasma (n=4) 89 84-94  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μU/ml OD1 OD2 Average    
    6000 0.089 0.084 0.087    
    3000 0.246 0.265 0.256    
    1500 0.551 0.580 0.566    
    750 0.933 0.981 0.957    
    375 1.646 1.569 1.608    
    0 2.362 2.398 2.380    
  • 数据处理：
  • 货期：
    3-5 working days

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