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  4. Fish Cholecystokinin(CCK) ELISA Kit

Fish Cholecystokinin(CCK) ELISA Kit

  • 中文名称：
    鱼胆囊收缩素/肠促胰酶肽(CCK)酶联免疫试剂盒
  • 货号：
    CSB-E13054Fh
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Fish CCK ELISA Kit was designed for the quantitative measurement of Fish CCK protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 200 pg/mL-5000 pg/mL and the sensitivity is 125 pg/mL.

  • 缩写：
    CCK
  • Uniprot No.：
  • 种属：
    Fish
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    200 pg/mL-5000 pg/mL
  • 灵敏度：
    125 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<15%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of fish CCK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %95
    Range %86-105
    1:2Average %89
    Range %84-96
    1:4Average %96
    Range %91-102
    1:8Average %93
    Range %86-105
  • 回收率：
    The recovery of fish CCK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8885-91
    EDTA plasma (n=4)9586-104
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2Average
    50000.137 0.135 0.136
    20000.241 0.228 0.235
    12000.324 0.339 0.332
    5000.616 0.577 0.597
    2001.235 1.274 1.255
    02.602 2.696 2.649
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

Fish CCk ELISA kit cat.numb
Could you give me the specific size parameters of the plate wells? As she will need it for the plate reader machine.

A:
Thanks for your inquiry.
The kit size is 96T and it is 8wells× 12 strips.Pls check the size:
W：8.5cm
L：12.5cm
X：1.25cm
Y：1cm
Generally the microplate reader with 450nm wave can be used. Our company uses the reader from Thermo MK-3.Pls let me know if you have any further questions. Thank you.