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Fish Catalase (CAT) ELISA kit

  • 中文名称：
    鱼过氧化氢酶(CAT)酶联免疫试剂盒
  • 货号：
    CSB-E15928Fh
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：
        CAT (Catalase)是一种抗氧化酶，负责将过氧化氢分解为氧气和水。CAT广泛分布于生物体内，尤其是在肝脏、肺部和红血球中含量较高。
        j9九游会登录入口首页生物所提供的Fish Catalase (CAT) ELISA kit属于ELISA检测试剂盒，采用竞争法定量检测鱼类血清、血浆样本中的CAT，其灵敏度为62.5 mU/mL，检测范围为125 mU/mL-2000 mU/mL。
     
  • 缩写：
  • Uniprot No.：
  • 种属：
    Fish
  • 样本类型：
    serum, plasma
  • 检测范围：
    125 mU/mL-2000 mU/mL
  • 灵敏度：
    62.5 mU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of fish CAT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:100 Average % 95  
    Range % 91-99  
    1:200 Average % 103  
    Range % 99-107  
    1:400 Average % 98  
    Range % 93-105  
    1:800 Average % 92  
    Range % 87-95  
  • 回收率：
    The recovery of fish CAT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 99 94-104  
    EDTA plasma (n=4) 88 83-96  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mU/ml OD1 OD2 Average    
    2000 0.091 0.089 0.090    
    1000 0.242 0.257 0.250    
    500 0.553 0.587 0.570    
    250 0.913 0.980 0.947    
    125 1.647 1.563 1.605    
    0 2.381 2.319 2.350    
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

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靶点详情

  • 最新研究进展：
    CAT是一种重要的抗氧化酶，在机体中起到清除自由基的作用，对细胞和组织的损伤具有保护作用。最新研究表明，CAT在代谢性疾病、肿瘤和神经系统等多个疾病的发生和发展中发挥着重要作用，通过抑制细胞凋亡、调节免疫反应等多种机制发挥保护作用，成为疾病治疗的潜在靶点。