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  4. Fish Acetylcholinesterase(AChE)ELISA Kit

Fish Acetylcholinesterase(AChE)ELISA Kit

  • 中文名称：
    鱼乙酰胆碱酯酶(AChE)酶联免疫试剂盒
  • 货号：
    CSB-E17001Fh
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Fish AChE ELISA Kit was designed for the quantitative measurement of Fish AChE protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 2.5 ng/mL-40 ng/mL and the sensitivity is 2.5 ng/mL.

  • 别名：
    acheAcetylcholinesterase ELISA kit; AChE ELISA kit; EC 3.1.1.7 ELISA kit
  • 缩写：
    AChE
  • Uniprot No.：
  • 种属：
    Fish
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    2.5 ng/mL-40 ng/mL
  • 灵敏度：
    2.5 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<15%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of fish AChE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 94
    Range % 89-103
    1:2 Average % 92
    Range % 87-101
    1:4 Average % 93
    Range % 87-100
    1:8 Average % 89
    Range % 83-99
  • 回收率：
    The recovery of fish AChE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 89 84-93
    EDTA plasma (n=4) 94 87-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average 　
    40 0.284 0.292 0.288 　
    20 0.521 0.532 0.527 　
    10 0.886 0.891 0.889 　
    5 1.374 1.382 1.378 　
    2.5 2.583 2.599 2.591 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. May be involved in cell-cell interactions.
  • 亚细胞定位：
    [Isoform H]: Cell membrane; Lipid-anchor, GPI-anchor. Cell junction, synapse.; [Isoform T]: Cell membrane; Peripheral membrane protein. Cell junction, synapse. Note=Attached to the membrane through disulfide linkage with the collagenic subunit, itself bound to the membrane.
  • 蛋白家族：
    Type-B carboxylesterase/lipase family
  • 组织特异性：
    Found in the synapses and to a lower extent in extrajunctional areas of muscle and nerve, and on erythrocyte membranes.