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Dog von Willebrand Factor,vWF ELISA Kit

  • 中文名称：
    犬血管性血友病因子/瑞斯托霉素辅因子(VWF)酶联免疫试剂盒
  • 货号：
    CSB-E12759c
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Dog VWF ELISA Kit was designed for the quantitative measurement of Dog VWF protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.32 ng/mL.

  • 别名：
    VWF ELISA Kit; F8VWF ELISA Kit; von Willebrand factor ELISA Kit; vWF ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.625 ng/mL-40 ng/mL
  • 灵敏度：
    0.32 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of dog vWF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:100 Average % 101  
    Range % 95-107  
    1:200 Average % 94  
    Range % 90-100  
    1:400 Average % 98  
    Range % 94-102  
    1:800 Average % 88  
    Range % 84-93  
  • 回收率：
    The recovery of dog vWF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 89-99  
    EDTA plasma (n=4) 97 90-101  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    40 0.093 0.101 0.097    
    20 0.209 0.216 0.213    
    10 0.422 0.441 0.432    
    5 0.739 0.715 0.727    
    2.5 1.168 1.124 1.146    
    1.25 1.602 1.709 1.656    
    0.625 1.779 1.879 1.829    
    0 2.170 2.297 2.234    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex, glycoprotein Ibalpha/IX/V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
  • 基因功能参考文献：
    1. only the c.1657G variant was found in the homozygous state exclusively in Von Willebrand disease affected dogs, and this variant is the strongest candidate to be the cause of VWD type 2 in the German Wirehaired Pointer and German Shorthaired Pointer breeds PMID: 28696025
    2. the CD-31 and vonWillebrand factor (vWF) expressions and the cell morphological alterations ofarachnoid villus endothelium in different stages of animal hydrocephalus PMID: 17593818
    3. Expression of mutant VWF proteins confirmed that VWF multimerization is not necessary for Weibel-Palade body creation PMID: 15331450
  • 相关疾病：
    Defects in VWF are the cause of von Willebrand disease (VWD) in the Scottish Terrier. VWD is characterized by frequent bleeding. Type I VWD is associated with a deficiency of VWF; type II by normal to decreased plasma level of VWF; type III by a virtual absence of VWF.
  • 亚细胞定位：
    Secreted. Secreted, extracellular space, extracellular matrix. Note=Localized to storage granules.
  • 组织特异性：
    Plasma.
  • 数据库链接：

    KEGG: cfa:399544

    STRING: 9615.ENSCAFP00000022453

    UniGene: Cfa.111