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  4. Dog motilin (MLN) ELISA kit

Dog motilin (MLN) ELISA kit

  • 中文名称：
    犬胃动素(MLN)酶联免疫试剂盒
  • 货号：
    CSB-EL014638DO
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Dog MLN ELISA Kit was designed for the quantitative measurement of Dog MLN protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 125 pg/mL-8000 pg/mL and the sensitivity is 31.25 pg/mL.

  • 别名：
    MLN ELISA Kit; Motilin ELISA Kit
  • 缩写：
    MLN
  • Uniprot No.：
  • 种属：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    125 pg/mL-8000 pg/mL
  • 灵敏度：
    31.25 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of dog MLN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 91  
    Range % 86-95  
    1:2 Average % 102  
    Range % 97-107  
    1:4 Average % 91  
    Range % 85-97  
    1:8 Average % 97  
    Range % 91-103  
  • 回收率：
    The recovery of dog MLN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 89-98  
    EDTA plasma (n=4) 97 90-100  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    8000 2.188 2.096 2.142 1.967  
    4000 1.487 1.406 1.447 1.272  
    2000 0.962 0.987 0.975 0.800  
    1000 0.626 0.607 0.617 0.442  
    500 0.434 0.468 0.451 0.276  
    250 0.361 0.335 0.348 0.173  
    125 0.292 0.281 0.287 0.112  
    0 0.171 0.178 0.175    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Plays an important role in the regulation of interdigestive gastrointestinal motility and indirectly causes rhythmic contraction of duodenal and colonic smooth muscle.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Motilin family
  • 数据库链接：