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  4. Dog matrix metalloproteinase 9/Gelatinase B,MMP-9 ELISA Kit

Dog matrix metalloproteinase 9/Gelatinase B,MMP-9 ELISA Kit

  • 中文名称：
    犬基质金属蛋白酶9/明胶酶B(MMP-9/Gelatinase B)酶联免疫试剂盒
  • 货号：
    CSB-E13887c
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Dog MMP9 ELISA Kit was designed for the quantitative measurement of Dog MMP9 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 12.5 ng/mL-800 ng/mL and the sensitivity is 3.12 ng/mL .

  • 别名：
    MMP9Matrix metalloproteinase-9 ELISA Kit; MMP-9 ELISA Kit; EC 3.4.24.35 ELISA Kit; 92 kDa gelatinase ELISA Kit; 92 kDa type IV collagenase ELISA Kit; Gelatinase B ELISA Kit; GELB ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 样本类型：
    serum, plasma
  • 检测范围：
    12.5 ng/mL-800 ng/mL
  • 灵敏度：
    3.12 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of dog MMP-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %99
    Range %92-105
    1:2Average %88
    Range %80-96
    1:4Average %87
    Range %85-90
    1:8Average %92
    Range %88-107
  • 回收率：
    The recovery of dog MMP-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8581-89
    EDTA plasma (n=4)9790-105
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    8002.096 2.076 2.086 1.977
    4001.513 1.605 1.559 1.450
    2001.135 1.118 1.127 1.018
    1000.618 0.655 0.637 0.528
    500.423 0.409 0.416 0.307
    250.301 0.278 0.290 0.181
    12.50.230 0.228 0.229 0.120
    00.110 0.107 0.109 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves NINJ1 to generate the Secreted ninjurin-1 form. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 蛋白家族：
    Peptidase M10A family
  • 数据库链接：