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  4. Dog Vascular Endothelial cell Growth Factor,VEGF ELISA KIT

Dog Vascular Endothelial cell Growth Factor,VEGF ELISA KIT

  • 中文名称：
    犬血管内皮细胞生长因子(VEGF)酶联免疫试剂盒
  • 货号：
    CSB-E11744c
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Dog VEGFA ELISA Kit was designed for the quantitative measurement of Dog VEGFA protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL.

  • 别名：
    VEGFA ELISA Kit; VEGF ELISA Kit; Vascular endothelial growth factor A ELISA Kit; VEGF-A ELISA Kit; Vascular permeability factor ELISA Kit; VPF ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    3.12 pg/mL-200 pg/mL
  • 灵敏度：
    0.78 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of dog VEGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %93
    Range %88-97
    1:2Average %101
    Range %97-106
    1:4Average %87
    Range %85-94
    1:8Average %91
    Range %84-96
  • 回收率：
    The recovery of dog VEGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9186-93
    EDTA plasma (n=4)8886-92
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    2003.076 3.050 3.063 2.964
    1002.538 2.517 2.528 2.429
    501.984 1.912 1.948 1.849
    251.203 1.228 1.216 1.117
    12.50.671 0.679 0.675 0.576
    6.250.308 0.317 0.313 0.214
    3.120.167 0.160 0.164 0.065
    00.101 0.097 0.099
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development.
  • 基因功能参考文献：
    1. Studied VEGF gene and protein expression as biomarker of canine mammary tumors (CMT). High VEGF levels were associated with malignant tumors compared with normal mammary glands. No difference in VEGF levels was detected between benign and malignant tumors or between nonmetastatic and metastatic carcinomas. PMID: 27543935
    2. Increased iNOS, COX-2 and VEGF gene mRNA levels were found to be related with the histological grade of malignancy in dogs with mammary tumours. PMID: 28956485
    3. Both VEGF and VEGFR-2 immunoreactivities were independent of patients' overall survival (OS) and disease-free survival (DFS). PMID: 24976305
    4. Yolk sac cells treated with VEGF had an increased cell growth rate in culture when compared with untreated cells. PMID: 26850540
    5. The effects of VEGF on folliculogenesis and its expression in cultured dog ovaries are reported. PMID: 25041787
    6. Lymphoma dogs showed higher act-MMP-9 and VEGF, and lower TGF-beta than controls, and a positive correlation between act-MMP-9 and VEGF. PMID: 22489798
    7. The morphology and expression pattern of VEGF and Ki-67 factors in corpus lutea of cyclic and gestational diestrous bitches were similar but significantly lower than that of late pregnant bitches. PMID: 23279467
    8. This study demonstrates that MMP-9, TIMP-2 and VEGF-A expression is related to histological grade in canine mast cell tumors. PMID: 22520817
    9. Although VEGF is involved in angiogenesis and correlates with tumour stage of development, no correlation was found between VEGF expression and microvessel density in canine renal cell carcinoma. PMID: 22300704
    10. Aqueous humor values of VEGF in diabetic dogs are not greater than nondiabetics and may serve to protect the dog against development of diabetic retinopathy. PMID: 21366824
    11. VEGF expression occurs in both benign and malignant mammary tumours and is independent of histological type, proliferation, tissue invasion or local metastatic capacity. PMID: 20570280
    12. altering the ferritin H:L ratio caused significant changes in iron uptake and storage, the rate of ferritin synthesis and degradation, the secretion of VEGF, and the levels of glutathione in cultured lens epithelial cells PMID: 20805568
    13. The expression of vascular endothelial growth factor (VEGF) and prostaglandin E2 (PGE-2) in 53 canine cutaneous mast cell tumours is reported. PMID: 20230578
    14. Data suggest that VEGF from P-APR might be a novel useful circulating bio-marker of tumor angiogenesis. PMID: 20001231
    15. The results of this study suggest that VEGF participates in the angiogenesis of soft-tissue sarcoma in dogs. Additional research will be necessary to elucidate the contribution of VEGF to the progression of malignancy. PMID: 20093695
    16. VEGF concentrations for all dogs after correcting for age, endostatin, and disease categories were associated with increased white blood cell count (P = .0032) and platelet counts (P = .0064) and decreased PCV (P = .0017). PMID: 16496934
    17. Expression of vascular endothelial growth factor (VEGF), its receptors (flt-1 and flk-1), and basic fibroblast growth factor (bFGF) in canine hemangiosarcoma (HSA) and hemangiomas was investigated by immunohistochemical analysis. PMID: 17099154
    18. VEGF protein was expressed by 60% of the tumours with diffuse cytoplasmic labelling of the neoplastic cells. Endothelial cells, macrophages and plasma cells were also immunolabelled. PMID: 17467003
    19. This study showed that, as an increase in tumor angiogenesis was observed in lymphoma samples compared to normal canine lymph node tissue, additional anti-angiogenic therapy, besides conventional chemotherapy as a lymphoma treatment may be effective. PMID: 17888471
    20. Early pulmonary hypertension in heart failure is characterized by altered vasoreactivity and increased ET-1 and VEGF2 signalling. PMID: 17993509
    21. The expression of markers association with angiogenesis was studied in canine squamous cell carcinomas and trichoepitheliomas. PMID: 18039895
    22. VEGF might be secreted from mast cells and it may be a suitable surrogate inter-species angiogenetic markers of tumour progression in canine cutaneous mast cell tumour . PMID: 18429933
    23. The relationship between the expression of the PTEN and VEGF and the clinicopathological features in canine mammary gland tumours are reported. PMID: 18461467
    24. Adipose tissue-derived mesenchymal stem cells expressed the mRNA of transforming growth factor beta (TGF-beta), IL-6, IL-8, CCL2, CCL5, VEGF,HGF, tissue inhibitor metalloproteinase-1/2, and cyclooxygenase-2 but not that of IL-10. PMID: 18717642
    25. VEGF and flt-1 are closely associated in canine, as in human tissues, and quantifying their mRNAs might be helpful in evaluating angiogenesis. PMID: 19057151
    26. Because VEGF expression is associated with survival, angiogenesis may be a more important predictor of canine meningioma activity than Ki-67. PMID: 19175733

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    PDGF/VEGF growth factor family
  • 数据库链接：