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Dog Orexin(HCRT) ELISA kit

  • 中文名称：
    犬阿立新(HCRT)酶联免疫试剂盒
  • 货号：
    CSB-EL010230DO
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Dog HCRT ELISA Kit was designed for the quantitative measurement of Dog HCRT protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.87 pg/mL-120 pg/mL and the sensitivity is 0.47 pg/mL.

  • 别名：
    HCRT ELISA Kit; OX ELISA Kit; PPOX ELISA Kit; Orexin ELISA Kit; Hypocretin ELISA Kit; Hcrt) [Cleaved into: Orexin-A ELISA Kit; Hypocretin-1 ELISA Kit; Hcrt1); Orexin-B ELISA Kit; Hypocretin-2 ELISA Kit; Hcrt2)] ELISA Kit
  • 缩写：
    HCRT
  • Uniprot No.：
  • 种属：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    1.87 pg/mL-120 pg/mL
  • 灵敏度：
    0.47 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of dog HCRT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 103
    Range % 96-107
    1:2 Average % 101
    Range % 98-105
    1:4 Average % 94
    Range % 89-98
    1:8 Average % 103
    Range % 96-107
  • 回收率：
    The recovery of dog HCRT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 91 89-95
    EDTA plasma (n=4) 103 98-107
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    120 2.176 2.234 2.205 2.101
    60 1.659 1.765 1.712 1.608
    30 1.193 1.223 1.208 1.104
    15 0.793 0.776 0.785 0.681
    7.5 0.492 0.483 0.488 0.384
    3.75 0.341 0.356 0.349 0.245
    1.87 0.242 0.249 0.246 0.142
    0 0.103 0.104 0.104 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. Orexin-A binds to both OX1R and OX2R with a high affinity, whereas orexin-B binds only to OX2R with a similar high affinity.
  • 基因功能参考文献：
    1. The presence of orexin A in the ovary either in dog or in cat is in agreement with previous data on the presence and role of orexins in the female genital system of other species. PMID: 25601132
  • 亚细胞定位：
    Rough endoplasmic reticulum. Cytoplasmic vesicle. Cell junction, synapse.
  • 蛋白家族：
    Orexin family
  • 数据库链接：