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Dog Matrix metalloproteinase 3,MMP-3 ELISA kit

  • 中文名称：
    犬基质金属蛋白酶3(MMP-3)酶联免疫试剂盒
  • 货号：
    CSB-E13621c
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Dog MMP3 ELISA Kit was designed for the quantitative measurement of Dog MMP3 protein in serum, plasma, cell culture supernates, urine, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL .

  • 别名：
    MMP3Stromelysin-1 ELISA Kit; SL-1 ELISA Kit; EC 3.4.24.17 ELISA Kit; Matrix metalloproteinase-3 ELISA Kit; MMP-3 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 样本类型：
    serum, plasma, cell culture supernates, urine, tissue homogenates
  • 检测范围：
    1.56 ng/mL-100 ng/mL
  • 灵敏度：
    0.39 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of dog MMP-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %92
    Range %88-96
    1:2Average %89
    Range %85-96
    1:4Average %93
    Range %89-97
    1:8Average %99
    Range %92-105
  • 回收率：
    The recovery of dog MMP-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9591-99
    EDTA plasma (n=4)9287-96
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    1002.410 2.545 2.478 2.284
    501.960 1.982 1.971 1.777
    251.595 1.563 1.579 1.385
    12.51.177 1.201 1.189 0.995
    6.250.786 0.789 0.788 0.594
    3.120.458 0.462 0.460 0.266
    1.560.278 0.262 0.270 0.076
    00.193 0.195 0.194 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
  • 基因功能参考文献：
    1. Findings suggested that increased TGF-beta3 and MMP-3 contribute to the pathogenesis of valvular degeneration associated with chronic mitral valve disease. PMID: 21281193
    2. Both mRNA transcript levels and the activities of MMP-3 and MMP-7 are elevated in X-linked Alport syndrome (XLAS) kidneys from dogs. PMID: 15782307
  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 蛋白家族：
    Peptidase M10A family
  • 数据库链接：