Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Dog Interleukin 18,IL-18 ELISA KIT

Dog Interleukin 18,IL-18 ELISA KIT

  • 中文名称：
    犬白介素18(IL-18)酶联免疫试剂盒
  • 货号：
    CSB-E09848c
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Dog IL18 ELISA Kit was designed for the quantitative measurement of Dog IL18 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 pg/mL-800 pg/mL and the sensitivity is 3.12 pg/mL.

  • 别名：
    IL18 ELISA Kit; IGIF ELISA Kit; Interleukin-18 ELISA Kit; IL-18 ELISA Kit; Interferon gamma-inducing factor ELISA Kit; IFN-gamma-inducing factor ELISA Kit; Interleukin-1 gamma ELISA Kit; IL-1 gamma ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    12.5 pg/mL-800 pg/mL
  • 灵敏度：
    3.12 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %104
    Range %100-109
    1:2Average %97
    Range %91-105
    1:4Average %90
    Range %85-94
    1:8Average %93
    Range %86-98
  • 回收率：
    The recovery of dog IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9589-99
    EDTA plasma (n=4)8780-91
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    8002.675 2.552 2.614 2.439
    4002.065 2.076 2.071 1.896
    2001.372 1.424 1.398 1.223
    1000.726 0.739 0.733 0.558
    500.471 0.504 0.488 0.313
    250.380 0.359 0.370 0.195
    12.50.271 0.259 0.265 0.090
    00.173 0.177 0.175
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    A proinflammatory cytokine primarily involved in polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses. Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells.
  • 亚细胞定位：
    Cytoplasm. Secreted.
  • 蛋白家族：
    IL-1 family
  • 数据库链接：

    KEGG: cfa:403796

    STRING: 9615.ENSCAFP00000038185

    UniGene: Cfa.40