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Dog Interleukin 10,IL-10 ELISA Kit

  • 中文名称：
    犬白介素10(IL-10)酶联免疫试剂盒
  • 货号：
    CSB-E11261c
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    This dog IL10 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of dog IL10 in multiple samples, including serum, plasma, or tissue homogenates. It also uses the enzyme-substrate chromogenic reaction to visualize and analyze the analyte levels through the color intensity. The intensity of the colored product is in direct proportion to the IL10 levels in the sample and is measured at 450 nm through a microplate reader.

    IL10 is an anti-inflammatory cytokine that is produced by many hematopoietic cells and mainly functions to inhibit macrophage and dendritic cell (DC) functions, which are necessary for the capture, control, and initiation of immune responses to pathogens. IL10 both impedes pathogen removal and improves immunopathology. IL-10 suppresses the expression of cytokines such as TNF-α, IL-1β, and IL-8, and can repress the expression of adhesion molecules. In addition, IL-10 can deactivate monocytes by downregulating the expression of MHC surface molecules. IL10 also plays a role in the control of allergy and asthma.

  • 别名：
    IL10 ELISA Kit; Interleukin-10 ELISA Kit; IL-10 ELISA Kit; Cytokine synthesis inhibitory factor ELISA Kit; CSIF ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    1.56 pg/mL-100 pg/mL
  • 灵敏度：
    0.39 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %97
    Range %92-108
    1:2Average %97
    Range %91-105
    1:4Average %89
    Range %84-96
    1:8Average %92
    Range %87-98
  • 回收率：
    The recovery of dog IL-10spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9085-95
    EDTA plasma (n=4)9287-96
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    1002.954 2.967 2.961 2.853
    502.388 2.398 2.393 2.285
    251.758 1.786 1.772 1.664
    12.51.035 1.109 1.072 0.964
    6.250.502 0.546 0.524 0.416
    3.120.271 0.267 0.269 0.161
    1.560.184 0.196 0.190 0.082
    00.112 0.103 0.108 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

If using this kit it is necessary to perform a a blood clotting time of 2 hours or overnight at 4 ° C and 15 minutes of centrifugation, and if so what is the reason for this procedure.

A:
Thanks for your inquiry.
It is the sample preparation method for serum indeed.
It is fine to do as the you mentioned for serum. Here also attached our manual for your checking.
The sample preparation method is through our our experimental experience and get it to prepare relatively large amounts of serum samples with relatively simple components.Pls let me know if you have any further questions. Thank you.

靶点详情

  • 功能：
    Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation. In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling.
  • 基因功能参考文献：
    1. seven new genetic variations of the IL10 gene were identified. PMID: 24487092
    2. Data indicate that the Leishmania braziliensis antigens plus saponin (LBSap) vaccine induced high levels of IL-12, IL-10, CCL4, CCL5 and CXCL8 expression within 48 hours. PMID: 23911411
    3. serum and tumor levels of IL-8 and IL-10 in dogs bearing benign and malignant mammary tumors, including dogs with inflammatory mammary cancer, for a better understanding of this disease PMID: 23351639
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    IL-10 family
  • 数据库链接：

    KEGG: cfa:403628

    STRING: 9615.ENSCAFP00000016812

    UniGene: Cfa.38