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  4. Dog Erythropoietin,EPO ELISA Kit

Dog Erythropoietin,EPO ELISA Kit

  • 中文名称：
    犬红细胞生成素(EPO)酶联免疫试剂盒
  • 货号：
    CSB-E11303c
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Dog EPO ELISA Kit was designed for the quantitative measurement of Dog EPO protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 mIU/mL-100 mIU/mL and the sensitivity is 0.39 mIU/mL.

  • 别名：
    EPOErythropoietin ELISA kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    1.56 mIU/mL-100 mIU/mL
  • 灵敏度：
    0.39 mIU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of dog EPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 97
    Range % 91-105
    1:2 Average % 89
    Range % 80-97
    1:4 Average % 93
    Range % 86-100
    1:8 Average % 95
    Range % 88-100
  • 回收率：
    The recovery of dog EPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 97 83-102
    EDTA plasma (n=4) 95 85-102
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mIU/ml OD1 OD2 Average Corrected
    100 1.902 1.893 1.898 1.725
    50 1.505 1.518 1.512 1.339
    25 1.087 1.146 1.117 0.944
    12.5 0.804 0.789 0.797 0.624
    6.25 0.499 0.512 0.506 0.333
    3.12 0.342 0.351 0.347 0.174
    1.56 0.255 0.261 0.258 0.085
    0 0.170 0.176 0.173 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3.
  • 基因功能参考文献：
    1. Developmental and compensatory lung growth involve paracrine erythropoietin signaling with parallel upregulation but differential processing of the erythropoietin receptor. PMID: 15286000
    2. apical secretion of EPO involves a cholesterol-dependent mechanism probably not involving lipid rafts PMID: 15916748
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    EPO/TPO family
  • 组织特异性：
    Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.
  • 数据库链接：