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Dog C-C motif chemokine 5(CCL5) ELISA kit

  • 中文名称：
    犬CC趋化因子5(CCL5)酶联免疫试剂盒
  • 货号：
    CSB-EL004800DO
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：
        CCL5，也称为RANTES（Regulated on Activation, Normal T cell Expressed and Secreted），是C-C趋化因子家族中的一种趋化因子。它由多种细胞产生，如T细胞、巨噬细胞、内皮细胞和平滑肌细胞等，并且能够吸引多种CCR1、CCR3和CCR5受体表达的细胞，如单核细胞、T细胞和巨噬细胞等。CCL5在多种炎症和免疫调节过程中发挥着重要作用，如艾滋病、动脉粥样硬化、炎症性肠病等。
        j9九游会登录入口首页生物所提供的Dog C-C motif chemokine 5(CCL5) ELISA kit属于ELISA检测试剂盒，采用双抗夹心法定量检测狗血清、血浆、组织匀浆样本中的CCL5，其灵敏度为0.081 ng/mL，检测范围为0.156 ng/mL-10 ng/mL。
     
  • 别名：
    CCL5 ELISA kit; SCYA5C-C motif chemokine 5 ELISA kit; Small-inducible cytokine A5 ELISA kit; T-cell-specific protein RANTES ELISA kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.081 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of dog CCL5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 105    
    Range % 97-110    
    1:2 Average % 101    
    Range % 95-106    
    1:4 Average % 96    
    Range % 87-101    
    1:8 Average % 102    
    Range % 97-107    
  • 回收率：
    The recovery of dog CCL5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 104 96-108    
    EDTA plasma (n=4) 99 91-104    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average Corrected   CV
    10 2.658 2.600 2.629 2.510   1.56%
    5 1.985 2.016 2.001 1.882   1.10%
    2.5 1.348 1.369 1.359 1.240   1.09%
    1.25 0.919 0.927 0.923 0.804   0.61%
    0.625 0.502 0.523 0.513 0.394   2.89%
    0.313 0.294 0.314 0.304 0.185   4.65%
    0.156 0.212 0.219 0.216 0.097   2.29%
    0 0.117 0.121 0.119 　   2.38%
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 最新研究进展：
        CCL5（C-C motif ligand 5），也称为RANTES（Regulated on Activation, Normal T Expressed and Secreted），是一种由多种细胞产生的细胞因子，包括T细胞、巨噬细胞和上皮细胞。CCL5在多种疾病中发挥重要作用，如炎症、艾滋病毒感染、自身免疫疾病和肿瘤。最近的研究表明，CCL5不仅在炎症和免疫过程中发挥作用，还可以促进肿瘤生长、侵袭和转移。
     
  • 功能：
    Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. May also be an agonist of the G protein-coupled receptor GPR75. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells.
  • 基因功能参考文献：
    1. Data indicate that the Leishmania braziliensis antigens plus saponin (LBSap) vaccine induced high levels of IL-12, IL-10, CCL4, CCL5 and CXCL8 expression within 48 hours. PMID: 23911411
    2. Adipose tissue-derived mesenchymal stem cells expressed the mRNA of transforming growth factor beta (TGF-beta), IL-6, IL-8, CCL2, CCL5, VEGF,HGF, tissue inhibitor metalloproteinase-1/2, and cyclooxygenase-2 but not that of IL-10. PMID: 18717642
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine beta (chemokine CC) family
  • 数据库链接：

    KEGG: cfa:403522

    STRING: 9615.ENSCAFP00000026835

    UniGene: Cfa.21