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Chicken Leptin,LEP ELISA kit

  • 中文名称：
    鸡瘦素(LEP)酶联免疫试剂盒
  • 货号：
    CSB-E14082C
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Chicken LEP ELISA Kit was designed for the quantitative measurement of Chicken LEP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

  • 别名：
    LEP ELISA Kit; OB ELISA Kit; Leptin ELISA Kit; Obesity factor ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Gallus gallus (Chicken)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of chicken LEP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %92
    Range %86-101
    1:2Average %95
    Range %85-104
    1:4Average %91
    Range %87-100
    1:8Average %93
    Range %89-106
  • 回收率：
    The recovery of chicken LEP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9786-107
    EDTA plasma (n=4)9588-99
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    202.540 2.472 2.506 2.373
    101.879 1.899 1.889 1.756
    51.138 1.123 1.131 0.998
    2.50.731 0.717 0.724 0.591
    1.250.411 0.402 0.407 0.274
    0.6250.308 0.315 0.312 0.179
    0.3120.200 0.209 0.205 0.072
    00.131 0.135 0.133
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Key player in the regulation of energy balance and body weight control. Once released into the circulation, has central and peripheral effects by binding LEPR, found in many tissues, which results in the activation of several major signaling pathways.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Leptin family
  • 组织特异性：
    Not exclusively localized in adipose tissue but is also expressed in liver.