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  4. Chicken Interleukin 8(IL-8) ELISA Kit

Chicken Interleukin 8(IL-8) ELISA Kit

  • 中文名称：
    鸡白介素8(IL-8)酶联免疫试剂盒
  • 货号：
    CSB-E14191C
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Chicken Interleukin 8 (IL-8) ELISA Kit is used for detecting and quantifying IL-8 protein in your chicken samples. IL-8,a chemokine involved in inflammatory responses and infection,plays a crucial role in the immune system. Our ELISA kit is designed specifically for the detection of IL-8 in serum, plasma, and tissue homogenates of Gallus gallus (chicken) samples.

    With a detection range of 1.56 pg/mL-400 pg/mL and a sensitivity of 0.78 pg/mL, this ELISA kit offers precise and accurate measurement of IL-8 levels in your samples. The assay principle is quantitative and competitive, making it highly specific and reliable. Assay time ranges from 1-5 hours, depending on the sample volume used (50-100ul), and detection wavelength is set at 450 nm. This kit is ideal for researchers in the field of immunology, as it can help in understanding the role of IL-8 in the immune response of chickens.

    The Chicken IL-8 ELISA Kit has been validated and cited in more than 6 research papers, making it a trusted tool for your research needs.

  • 别名：
    CXCL8 ELISA Kit; CEF4 ELISA Kit; EMF1 ELISA Kit; IL8 ELISA Kit; Interleukin-8 ELISA Kit; IL-8 ELISA Kit; 9E3 ELISA Kit; C-X-C motif chemokine 8 ELISA Kit; CEF-4 ELISA Kit; Chemokine ELISA Kit; C-X-C motif) ligand 8 ELISA Kit; Embryo fibroblast protein 1 ELISA Kit; EMF-1 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Gallus gallus (Chicken)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    1.56 pg/mL-400 pg/mL
  • 灵敏度：
    0.78 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 102  
    Range % 97-106  
    1:2 Average % 88  
    Range % 84-93  
    1:4 Average % 104  
    Range % 99-109  
    1:8 Average % 103  
    Range % 99-106  
  • 回收率：
    The recovery of chicken IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 98 94-102  
    EDTA plasma (n=4) 91 85-96  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    400 0.187 0.183 0.185    
    100 0.259 0.267 0.263    
    25 0.471 0.452 0.462    
    6.25 0.862 0.843 0.853    
    1.56 1.410 1.398 1.404    
    0 2.251 2.159 2.205    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

Can I get a copy of the kit instructions to as well as any references to papers that have used this kit? I want to make sure it will work in our specific use case. To that end I'm looking for information about the nature of the antibodies (capture and detection) are they monoclonal or poly. Are they the same Ab for both steps or different? Are the plates recoated, or do we need to coat them ourselves? Thank you for the help.

A:
Thanks for your inquiry.
I will send you the manual by e-mail.
Here is the reference about this kit: https://www.sciencedirect.com/science/article/pii/S0165242717302362
The plate is ready to use and you don't need to coat it by yourself. The plate is coated with specific antigen.There is only one antibody in the kit and it is goat polyclonal antibody.
Pls let me know if you have any further questions. Thank you.

靶点详情

  • 功能：
    May be an autocrine factor that promotes the growth of fibroblasts and is involved in the neoplastic transformation of fibroblasts by v-Src. Chemotactic for peripheral blood mononuclear cells as well as for heterophils.
  • 基因功能参考文献：
    1. Lower levels in airway aspirates from breastfed infants with acute bronchiolitis PMID: 20337964
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine alpha (chemokine CxC) family
  • 数据库链接：

    KEGG: gga:396495

    STRING: 9031.ENSGALP00000019051

    UniGene: Gga.826