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Chicken Interleukin 6,IL-6 ELISA Kit

  • 中文名称：
    鸡白介素6(IL-6)酶联免疫试剂盒
  • 货号：
    CSB-E08549Ch
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：
        IL6（白细胞介素6, interleukin-6）是一种细胞因子，参与多种免疫和炎症反应。在感染和炎症等情况下，IL6的水平明显升高。
        j9九游会登录入口首页生物所提供的Chicken Interleukin 6,IL-6 ELISA Kit属于ELISA检测试剂盒，采用双抗夹心法定量检测鸡血清、血浆、组织匀浆样本中的IL6，其灵敏度为3.9 pg/mL，检测范围为15.6 pg/mL-1000 pg/mL。
     
  • 别名：
    IL6 ELISA Kit; Interleukin-6 ELISA Kit; ChIL-6 ELISA Kit; IL-6 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Gallus gallus (Chicken)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 93  
    Range % 85-97  
    1:2 Average % 92  
    Range % 85-99  
    1:4 Average % 95  
    Range % 89-100  
    1:8 Average % 92  
    Range % 85-100  
  • 回收率：
    The recovery of chicken IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 98 91-105  
    EDTA plasma (n=4) 96 88-99  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    1000 2.487 2.570 2.529 2.454  
    500 2.204 2.230 2.217 2.142  
    250 1.800 1.764 1.782 1.707  
    125 1.390 1.394 1.392 1.317  
    62.5 0.917 0.950 0.934 0.859  
    31.2 0.584 0.568 0.576 0.501  
    15.6 0.348 0.362 0.355 0.280  
    0 0.074 0.075 0.075 　  
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 最新研究进展：
    IL-6是一种多功能细胞因子，可以影响许多不同类型的细胞和器官。最新研究表明，IL-6参与了许多疾病的发病机制，如自身免疫性疾病、心血管疾病、肿瘤等。此外，IL-6在新冠疫情中也扮演了重要角色，成为疫苗研究和治疗的关键目标之一。针对IL-6的治疗方法，如抗IL-6受体抗体和抑制IL-6表达的药物等，在临床治疗中已经取得了一些成功。
  • 功能：
    Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway. The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells.
  • 基因功能参考文献：
    1. Avian leukosis virus subgroup J induces VEGF expression via NF-kappaB/PI3K-dependent IL-6 production to promoter tumorigenesis. PMID: 27852059
    2. The effects of daily corticosterone injections on acute phase proteins (APP), IL-6, and HSP70 are reported; APP and HSP70 may be of value as indicators of stress in poultry. PMID: 25306460
    3. Chicken IL-6 is a heat-shock gene PMID: 24055475
    4. Nampt/visfatin/PBEF affects expression of myogenic regulatory factors and is regulated by interleukin-6 in chicken skeletal muscle cells PMID: 21545843
    5. IL-6 is involved in the progression of chicken arthritis induced by Staph. aureus, and it contributes to disease incidence and mortality PMID: 17495100

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    IL-6 superfamily
  • 数据库链接：

    KEGG: gga:395337

    UniGene: Gga.2769