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  4. Chicken Glutathione Peroxidase 8 (GPX-8) ELISA Kit

Chicken Glutathione Peroxidase 8 (GPX-8) ELISA Kit

  • 中文名称：
    鸡谷胱甘肽过氧化物酶8(GPX-8)酶联免疫试剂盒
  • 货号：
    CSB-E14942C
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Chicken GPX-8 ELISA Kit was designed for the quantitative measurement of Chicken GPX-8 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 31.25 mU/mL-2000 mU/mL and the sensitivity is 7.8 mU/mL.

  • 缩写：
    GPX-8
  • Uniprot No.：
  • 种属：
    Gallus gallus (Chicken)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    31.25 mU/mL-2000 mU/mL
  • 灵敏度：
    7.8 mU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of chicken GPX-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:100 Average % 98    
    Range % 94-103    
    1:200 Average % 86    
    Range % 83-90    
    1:400 Average % 104    
    Range % 100-108    
    1:800 Average % 89    
    Range % 85-93    
  • 回收率：
    The recovery of chicken GPX-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 107 103-110    
    EDTA plasma (n=4) 84 80-88    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    mU/ml OD1 OD2 Average      
    2000 0.111 0.115 0.113      
    1000 0.208 0.210 0.209      
    500 0.373 0.390 0.382      
    250 0.642 0.638 0.640      
    125 0.956 0.961 0.959      
    62.5 1.146 1.200 1.173      
    31.25 1.417 1.431 1.424      
    0 1.634 1.645 1.640      
  • 数据处理：
  • 货期：
    3-5 working days

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