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Chicken Ghrelin ELISA Kit

  • 中文名称：
    鸡饥饿激素酶联免疫试剂盒
  • 货号：
    CSB-E14230C
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Chicken Ghrelin ELISA Kit was designed for the quantitative measurement of Chicken Ghrelin protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 25 pg/mL-400 pg/mL and the sensitivity is 25 pg/mL.

  • 缩写：
    Ghrelin
  • 种属：
    Gallus gallus (Chicken)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    25 pg/mL-400 pg/mL
  • 灵敏度：
    25 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of chicken ghrelin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 87-96  
    1:2 Average % 90  
    Range % 86-95  
    1:4 Average % 95  
    Range % 90-100  
    1:8 Average % 97  
    Range % 92-103  
  • 回收率：
    The recovery of chicken ghrelin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 90-98  
    EDTA plasma (n=4) 93 88-98  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    400 0.248 0.244 0.246    
    200 0.506 0.500 0.503    
    100 0.863 0.856 0.860    
    50 1.687 1.702 1.695    
    25 2.515 2.423 2.469    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 客户评论
  • Sample type: Plasma
    Sample species: Chicken
    Review: The assay was able to detect the differences between treatments.