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  4. Canine brain natriuretic peptide(BNP)ELISA Kit

Canine brain natriuretic peptide(BNP)ELISA Kit

  • 中文名称：
    犬脑钠素/脑钠尿肽(BNP)酶联免疫试剂盒
  • 货号：
    CSB-E17436c
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Dog BNP ELISA Kit was designed for the quantitative measurement of Dog BNP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 pg/mL-800 pg/mL and the sensitivity is 3.12 pg/mL.

  • 别名：
    NPPBNatriuretic peptides B ELISA kit; Gamma-brain natriuretic peptide) [Cleaved into: Brain natriuretic peptide 34 ELISA kit; BNP-34); Brain natriuretic peptide 29 ELISA kit; BNP-29)] ELISA kit
  • 缩写：
    BNP
  • Uniprot No.：
  • 种属：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    12.5 pg/mL-800 pg/mL
  • 灵敏度：
    3.12 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of canine BNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 95  
    Range % 90-105  
    1:2 Average % 91  
    Range % 88-96  
    1:4 Average % 95  
    Range % 90-102  
    1:8 Average % 97  
    Range % 92-103  
  • 回收率：
    The recovery of canine BNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 98 93-105  
    EDTA plasma (n=4) 96 92-101  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    800 1.867 1.953 1.910 1.706  
    400 1.524 1.600 1.562 1.358  
    200 1.166 1.124 1.145 0.941  
    100 0.812 0.862 0.837 0.633  
    50 0.524 0.563 0.544 0.340  
    25 0.375 0.401 0.388 0.184  
    12.5 0.278 0.259 0.269 0.065  
    0 0.203 0.204 0.204 　  
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Cardiac hormone that plays a key role in mediating cardio-renal homeostasis. May also function as a paracrine antifibrotic factor in the heart. Acts by specifically binding and stimulating NPR1 to produce cGMP, which in turn activates effector proteins that drive various biological responses. Involved in regulating the extracellular fluid volume and maintaining the fluid-electrolyte balance through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Binds the clearance receptor NPR3.; May affect cardio-renal homeostasis. Able to promote the production of cGMP although its potency is very low compared to brain natriuretic peptide 32.
  • 亚细胞定位：
    Secreted.; [Brain natriuretic peptide 32]: Secreted.
  • 蛋白家族：
    Natriuretic peptide family
  • 组织特异性：
    Brain and also in atria, but at much lower levels than ANP.
  • 数据库链接：