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  4. Canine S100 calcium binding protein A12/Calgranulin-C(S100A12) ELISA Kit

Canine S100 calcium binding protein A12/Calgranulin-C(S100A12) ELISA Kit

  • 中文名称：
    犬S100钙结合蛋白A12/钙粒蛋白C(S100A12)酶联免疫试剂盒
  • 货号：
    CSB-E17221c
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Dog S100A12 ELISA Kit was designed for the quantitative measurement of Dog S100A12 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

  • 缩写：
    S100A12
  • Uniprot No.：
  • 种属：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    0.625 ng/mL-40 ng/mL
  • 灵敏度：
    0.156 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of canine S100A12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %85
    Range %81-89
    1:2Average %96
    Range %93-99
    1:4Average %87
    Range %82-91
    1:8Average %97
    Range %91-103
  • 回收率：
    The recovery of canine S100A12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8985-93
    EDTA plasma (n=4)9790-104
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    401.928 2.035 1.982 1.853
    201.460 1.568 1.514 1.385
    100.949 0.928 0.939 0.810
    50.565 0.578 0.572 0.443
    2.50.377 0.398 0.388 0.259
    1.250.252 0.264 0.258 0.129
    0.6250.196 0.198 0.197 0.068
    00.132 0.126 0.129
  • 数据处理：
  • 货期：
    3-5 working days

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