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  4. Canine Relaxin(RLN) ELISA Kit

Canine Relaxin(RLN) ELISA Kit

  • 中文名称：
    犬松弛肽/松弛素(RLN)酶联免疫试剂盒
  • 货号：
    CSB-E14982c
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Dog RLN ELISA Kit was designed for the quantitative measurement of Dog RLN protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL.

  • 别名：
    RLN ELISA Kit; Prorelaxin [Cleaved into: Relaxin B chain; Relaxin A chain] ELISA Kit
  • 缩写：
    RLN
  • Uniprot No.：
  • 种属：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    3.12 pg/mL-200 pg/mL
  • 灵敏度：
    0.78 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of canine RLN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 87
    Range % 84-90
    1:2 Average % 95
    Range % 91-99
    1:4 Average % 88
    Range % 84-93
    1:8 Average % 97
    Range % 93-100
  • 回收率：
    The recovery of canine RLN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 104 101-107
    EDTA plasma (n=4) 99 94-103
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    200 2.366 2.504 2.435 2.269
    100 1.644 1.602 1.623 1.457
    50 0.901 0.964 0.933 0.767
    25 0.487 0.468 0.478 0.312
    12.5 0.319 0.327 0.323 0.157
    6.25 0.261 0.268 0.265 0.099
    3.12 0.206 0.196 0.201 0.035
    0 0.161 0.171 0.166 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Relaxin is an ovarian hormone that acts with estrogen to produce dilatation of the birth canal in many mammals.
  • 基因功能参考文献：
    1. the luteal expression and localization of the RLN system was investigated by immunohistochemistry using custom-made antibodies and semi-quantitative PCR, at selected time points during gestation PMID: 29364921
    2. The blood levels of multiple steroids and other hormones in adult male dogs with or without prostatic hyperplasia are reported. PMID: 23279510
    3. The expression of relaxin and its receptors in canine mammary neoplasm tissue were studied, indicating a possible significant role of relaxin as an inducer of connective tissue remodelling. PMID: 19754574
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Insulin family
  • 组织特异性：
    Placenta; syncytiotrophoblast.
  • 数据库链接：