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  4. Canine Parathyroid hormone(PTH) ELISA Kit

Canine Parathyroid hormone(PTH) ELISA Kit

  • 中文名称：
    犬甲状旁腺激素(PTH)酶联免疫试剂盒
  • 货号：
    CSB-E17575c
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Dog PTH ELISA Kit was designed for the quantitative measurement of Dog PTH protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

  • 别名：
    PTH ELISA Kit; Parathyroid hormone ELISA Kit; PTH ELISA Kit; Parathyrin ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of canine PTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 96
    Range % 86-102
    1:2 Average % 96
    Range % 90-103
    1:4 Average % 89
    Range % 83-102
    1:8 Average % 97
    Range % 92-105
  • 回收率：
    The recovery of caninePTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 94 82-105
    EDTA plasma (n=4) 96 90-102
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1000 2.704 2.721 2.713 2.592
    500 2.335 2.347 2.341 2.220
    250 1.976 1.991 1.984 1.863
    125 1.493 1.482 1.488 1.367
    62.5 0.997 0.969 0.983 0.862
    31.2 0.482 0.476 0.479 0.358
    15.6 0.373 0.364 0.369 0.248
    0 0.122 0.119 0.121 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.
  • 基因功能参考文献：
    1. Acute metabolic and respiratory alkalosis markedly decreased PTH values during normocalcemia and delayed the PTH response to hypocalcemia PMID: 12929937
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Parathyroid hormone family
  • 数据库链接：