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  4. Canine N-terminal pro-brain natriuretic peptide(NT-proBNP)ELISA Kit

Canine N-terminal pro-brain natriuretic peptide(NT-proBNP)ELISA Kit

  • 中文名称：
    犬N端前脑钠素(NT-proBNP)酶联免疫试剂盒
  • 货号：
    CSB-E17577c
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Dog NT-proBNP ELISA Kit was designed for the quantitative measurement of Dog NT-proBNP protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

  • 缩写：
    NT-proBNP
  • 种属：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 样本类型：
    serum, plasma
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of canine NT-proBNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 84  
    Range % 80-92  
    1:2 Average % 97  
    Range % 90-105  
    1:4 Average % 97  
    Range % 92-104  
    1:8 Average % 93  
    Range % 86-100  
  • 回收率：
    The recovery of canine NT-proBNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 85-108  
    EDTA plasma (n=4) 97 92-106  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    20 2.675 2.655 2.665 2.516  
    10 2.152 2.144 2.148 1.999  
    5 1.311 1.302 1.307 1.158  
    2.5 0.781 0.799 0.790 0.641  
    1.25 0.377 0.363 0.370 0.221  
    0.625 0.264 0.254 0.259 0.110  
    0.312 0.186 0.190 0.188 0.039  
    0 0.146 0.152 0.149 　  
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

Per customers request, please provide the following information regarding Canine N-terminal pro-brain natriuretic peptide, NT-proBNP ELISA Kit (CSB-E17577c)
Can this kit be used with cat samples too? Or is this kit only designed to be used for dogs?
1.What is the standard? (Recombinant full-length protein? Partial protein
(range?)?, peptide (sequence range?), native protein?)
2. If recombinant protein, then what is the host?
3. Is the capture antibody a monoclonal or polyclonal?
4. What is the host of the capture antibody?
5. What is the immunogen of the capture antibody?
6. Is the detection antibody a monoclonal or polyclonal?
7. What is the host of the detection antibody?
8. What is the immunogen of the detection antibody?

A:
Thanks for your inquiry!
CSB-E17577c
This kit is specific to dog samples. It is not suggested to be used to test other species.
Capture antibody is mouse monoclonal antibody and immunogen is 293 cell-derived recombinant protein(full length).
Detection antibody is goat polyclonal antibody and immunogen is 293 cell-derived recombinant protein(fragment).
Standard is 293 cell-derived recombinant protein. Sorry we can not disclose the sequence.
Pls let me know if you have any further questions. Thank you.