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Canine Interferon alpha-1/2 ELISA kit

  • 中文名称：
    犬α-1/2干扰素(IFN-α1/2)酶联免疫试剂盒
  • 货号：
    CSB-E09849c
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Dog IFN-A1/2 ELISA Kit was designed for the quantitative measurement of Dog IFN-A1/2 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 pg/mL-800 pg/mL and the sensitivity is 3.12 pg/mL.

  • 别名：
    Interferon alpha-1/2 ELISA Kit; IFN-alpha-1/2 ELISA Kit
  • 缩写：
    IFN-α1/2
  • Uniprot No.：
  • 种属：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    12.5 pg/mL-800 pg/mL
  • 灵敏度：
    3.12 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of canine IFN-α1/2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %95
    Range %86-99
    1:2Average %100
    Range %94-109
    1:4Average %92
    Range %89-96
    1:8Average %87
    Range %83-96
  • 回收率：
    The recovery of canine IFN-α1/2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8985-96
    EDTA plasma (n=4)9488-99
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    8002.815 2.824 2.820 2.726
    4002.417 2.425 2.421 2.327
    2001.834 1.863 1.849 1.755
    1001.042 1.112 1.077 0.983
    500.518 0.563 0.541 0.447
    250.248 0.269 0.259 0.165
    12.50.126 0.131 0.129 0.035
    00.091 0.096 0.094 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Alpha/beta interferon family
  • 数据库链接：

    KEGG: cfa:100685213

    UniGene: Cfa.4550