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Bovine luteinizing hormone (LH) ELISA kit

  • 中文名称:
    牛黄体生成素(LH)酶联免疫试剂盒
  • 货号:
    CSB-E12826B
  • 规格:
    96T/48T
  • 价格:
    ¥4200/¥3000
  • 其他:

产品详情

  • 产品描述:

    This Bovine luteinizing hormone (LH) ELISA Kit was designed for the quantitative measurement of Bovine luteinizing hormone (LH) protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 1.25 mIU/mL-100 mIU/mL and the sensitivity is 1.56 mIU/mL.

  • 别名:
    LHBLutropin subunit beta ELISA kit; Luteinizing hormone subunit beta ELISA kit; LH-B ELISA kit; LSH-B ELISA kit; LSH-beta ELISA kit; Lutropin beta chain ELISA kit
  • 缩写:
    LH
  • Uniprot No.:
  • 种属:
    Bos taurus (Bovine)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    1.25 mIU/mL-100 mIU/mL
  • 灵敏度:
    1.56 mIU/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Signal Transduction
  • 测定原理:
    quantitative
  • 测定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine LH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 99  
    Range % 95-102  
    1:2 Average % 87  
    Range % 84-90  
    1:4 Average % 88  
    Range % 83-94  
    1:8 Average % 100  
    Range % 95-105  
  • 回收率:
    The recovery of bovine LH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 93 90-95  
    EDTA plasma (n=4) 87 85-92  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mIU/ml OD1 OD2 Average    
    100 0.361 0.368 0.365    
    37.5 0.538 0.567 0.553    
    18.75 0.781 0.765 0.773    
    6.25 1.156 1.126 1.141    
    1.25 1.662 1.698 1.680    
    0 2.161 2.178 2.170    
  • 数据处理:
  • 货期:
    3-5 working days

产品评价

靶点详情

  • 功能:
    Promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids.
  • 亚细胞定位:
    Secreted.
  • 蛋白家族:
    Glycoprotein hormones subunit beta family
  • 数据库链接: