Bovine complement fragment 5a (C5a) ELISA kit

产品详情

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

This Bovine C5a ELISA Kit was designed for the quantitative measurement of Bovine C5a protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 4.69 ng/mL-300 ng/mL and the sensitivity is 1.17 ng/mL.
缩写：

C5a
种属：

Bos taurus (Bovine)
样本类型：

serum, plasma, tissue homogenates
检测范围：

4.69 ng/mL-300 ng/mL
灵敏度：

1.17 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine C5a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	88-93
1:2	Average %	101
	Range %	97-104
1:4	Average %	94
	Range %	88-99
1:8	Average %	93
	Range %	89-97

回收率：

The recovery of bovine C5a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-103
EDTA plasma (n=4)	90	86-94

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.