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  4. Bovine beta-lactoglobulin (Beta-LG) (allergen Bos d 5) (LGB)ELISA kit

Bovine beta-lactoglobulin (Beta-LG) (allergen Bos d 5) (LGB)ELISA kit

  • 中文名称：
    牛β-乳球蛋白(β- LG)(过敏原Bos d 5) (LGB)酶联免疫试剂盒
  • 货号：
    CSB-EL027168BO
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：
        LGB (磷脂酶B，Lysophosphatidylglycerol Acyltransferase 2) 是一种参与脂肪酸代谢的酶，将磷脂酰甘油酯化为二酰基甘油。LGB作为内质网膜上的磷脂酰转移酶，在细胞脂质代谢、信号转导和细胞凋亡等方面发挥重要作用。在人体内，LGB主要存在于肝脏、肠道和脾脏等组织中。其含量的变化可以反映肝细胞和肠道的脂质代谢水平。
        j9九游会登录入口首页生物所提供的Bovine beta-lactoglobulin (Beta-LG) (allergen Bos d 5) (LGB)ELISA kit属于ELISA检测试剂盒，采用竞争法定量检测牛血清、血浆、milk样本中的LGB，其灵敏度为0.129 μg/mL，检测范围为0.156 μg/mL-10 μg/mL。
     
  • 别名：
    Beta lactoglobulin B ELISA Kit; Beta-lactoglobulin ELISA Kit; Beta-LG ELISA Kit; BLG ELISA Kit; LACB_BOVIN ELISA Kit; LGB ELISA Kit
  • 缩写：
    LGB
  • Uniprot No.：
  • 种属：
    Bos taurus (Bovine)
  • 样本类型：
    serum, plasma, milk
  • 检测范围：
    0.156 μg/mL-10 μg/mL
  • 灵敏度：
    0.129 μg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine LGB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 95  
    Range % 87-101  
    1:2 Average % 92  
    Range % 90-95  
    1:4 Average % 89  
    Range % 85-93  
    1:8 Average % 95  
    Range % 90-98  
  • 回收率：
    The recovery of bovine LGB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 93-99  
    EDTA plasma (n=4) 94 89-100  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/ml OD1 OD2 Average    
    10 0.173 0.168 0.171    
    5 0.279 0.273 0.276    
    2.5 0.376 0.358 0.367    
    1.25 0.482 0.472 0.477    
    0.625 0.631 0.653 0.642    
    0.313 0.948 0.914 0.931    
    0.156 1.166 1.193 1.180    
    0 1.884 1.952 1.918    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 最新研究进展：
    LGB是一种蛋白质，全名为胆红素结合蛋白。它主要在肝脏中合成，并参与胆红素的转运和代谢。最近的研究表明，LGB还参与了一些疾病的发生和发展，如心血管疾病、糖尿病等。此外，LGB的一些基因变异也与肿瘤等疾病的风险相关。
  • 功能：
    Primary component of whey, it binds retinol and is probably involved in the transport of that molecule.
  • 基因功能参考文献：
    1. the conformational complexity of BLG protein-protein self-association and palmitic acid (PLM) or dodecyl sulfate (SDS) ligand recognition in the monomeric and dimeric state, was investigated. PMID: 27456117
    2. The effect of beta-lactoglobulin (beta-LG) phenotype on the fatty acid composition and antioxidant capacity of milk is variable, which could partly be the result of a beta-LG phenotype x diet interaction. PMID: 26174000
    3. LG plays a key role in enhancing immune responses by promoting cell proliferation through IgM receptor. PMID: 27957499
    4. Interactions of LGB-A and LGB-B with sodium dodecyl sulfate, dodecyltrimethylammonium chloride and lauric acid, 12-carbon ligands possessing differently charged polar groups, were investigated.[LGB-A, LGB-B] PMID: 23784992
    5. Data (including data from molecular dynamic simulations) suggest that -OH groups of 3-hydroxypalmitic acid and 16-hydroxypalmitic acid act as hydrogen-bond donors in a hydrophobic protein cavity of beta-lactoglobulin. [BLG, beta-lactoglobulin] PMID: 24658798
    6. SNPs at the exon2 region of the beta-LG gene affected milk performance traits in Chinese Holstein cattle. PMID: 22097730
    7. Rate constants (k(off)) for betaLg dimer dissociation, were estimated. PMID: 22853908
    8. Because of a more favorable enthalpy, the dimerization equilibrium constant of beta-LG B was larger than that of beta-LG A at room temperature, while the two dimers became similarly stable at 35 degrees C. PMID: 21117642
    9. Three single nucleotide polymorphisms change beta-LG B* gene structure and expression. PMID: 21750913
    10. Kinetic studies of the interaction of beta-lactoglobulin with model membranes: stopped-flow CD and fluorescence studies. PMID: 20822106
    11. multiple binding regions with a specific and different affinity for structurally different classes of hydrophobic drugs and, that bovine beta-lactoglobulin can bind and protect against low pH values various classes of drugs of pharmaceutical relevance. PMID: 19919177
    12. Studies show that the addition of salts to water at pH 3.0 favors the dimerization of beta-lactoglobulin, and the dimer is the dominant species at 1M NaCl, 1M GuHCl, and 25 mM NaClO(4). PMID: 19241465
    13. The stability of beta-lactoglobulin against unfolding is sharply affected by the pH of the medium: both A and B LGB variants are maximally stabilized against urea denaturation at acidic pH and against sodium dodecyl sulfate denaturation at alkaline pH. PMID: 12484782
    14. association between single nucleotide polymorphism in the activator protein-2 binding site of the beta-lactoglobulin gene and its gene product can be explained as the result of differences in protein binding activity PMID: 12836958
    15. In apo-beta-lactoglobulin simulations starting from the protonated crystal structure, a long-lived H-bond interaction between the protonated Glu89 and Ser116 was observed PMID: 14997570
    16. Results suggest that the stability of beta-lactoglobulin arising from the hydrophobic effect is reduced by the C121S mutation so that unfolded or partially unfolded states are more favored. PMID: 15147215
    17. PPIX binds a bLG site affected by the pH of the solution PMID: 16297563
    18. Data describe the Tanford transition, a conformational change of bovine beta-lactoglobulin (betaLG) occurring at around pH 7, using heteronuclear NMR. PMID: 16368109
    19. bovine beta-lactoglobulin (variant A) structural analysis at very low ionic strength PMID: 16540345
    20. Comparison among the various probes shows multiple binding sites for hydrophobes are present in the native beta-lactoglobulin (BLG)and in the partially-and reversibly-modified form of BLG present in solution at neutral pH and subdenaturing temperatures. PMID: 16721656
    21. the interaction and orientation of betaLG in association with various artificial lipid vesicles at neutral and acidic pH have been studied by use of several complementary spectroscopic techniques. PMID: 16819842
    22. Found a C to A transversion at position 215 bp upstream of the translation initiation site (g.-215C>A)that segregated perfectly with differential phenotypic expression in a paternal half-sib family. PMID: 17033029
    23. Beta lactoglobulin provides an example of binding with low specificity but high affinity, which may play an important role in protein-ligand and protein-protein networks. PMID: 17331535
    24. analysis of pH-dependent conformational transitions of bovine beta-lactoglobulin monitored by heteronuclear NMR PMID: 17878316
    25. the contributions of interface area desolvation and water immobilization accounted satisfactorily for the experimental heat capacity PMID: 17910061
    26. Human LIMR, known to act as an endocytic receptor for lipocalin-1, also binds bovine BLG and mediates its cellular uptake. PMID: 17991420
    27. A model reveals that one vitamin D3 molecule binds to the calyx (central internal binding-site) of beta-LG and the other binds to the surface of beta-LG between the alpha-helix and beta-strand I (external binding site). PMID: 18004750
    28. The three-dimensional structure of BLG complexed with Ige Fab fragments provides the first insight into IgE antibody-allergen interactions at the molecular level. PMID: 18097096
    29. In heterozygotes, beta-casein A and beta-lactoglobulin A were found at higher concentrations in milk compared with the variant encoded by the alternative allele whereas kappa-casein A and B variants were found at similar concentrations in AB heterozygotes PMID: 18363977
    30. The extreme case of a completely dehydrated free binding site is realized for the large nonpolar binding cavity in bovine beta-lactoglobulin. PMID: 18427121
    31. bovine beta-lactoglobulin dimers folding PMID: 18465840
    32. No differences were found in milk compositional characteristics among genetic variants of beta-lactoglobulin in 2 breeds of cows; no association between milk composition and beta-lactoglobulin was observed. PMID: 18474134
    33. Noncovalent labeling with thioflavin T and 1-anilino-8-naphthalenesulfonate was used to follow the conformational changes occurring in beta-lactoglobulin during aggregation using time resolved luminescence. PMID: 18590743
    34. the expression of soluble wild type recombinant BGL in Escherichia coli cells at about 109 mg rBLG/g wet weight cells and a comparison between the aggregation of wt BLG and its variant C121S upon intracellular expression. PMID: 18662787
    35. DNA methylation changes in cell line from beta-lactoglobulin gene targeted fetus. PMID: 18789616
    36. detected new polymorphisms in the beta-lactoglobulin (beta-LG) gene with significant effects on beta-LG protein concentration PMID: 19032698

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Calycin superfamily, Lipocalin family
  • 组织特异性：
    Synthesized in mammary gland and secreted in milk.
  • 数据库链接：

    KEGG: bta:280838

    STRING: 9913.ENSBTAP00000019538

    UniGene: Bt.385