Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Bovine anti-Mullerian hormone (AMH) ELISA kit

Bovine anti-Mullerian hormone (AMH) ELISA kit

  • 中文名称：
    牛缪勒管抑制物质/抗缪勒管激素(AMH)酶联免疫试剂盒
  • 货号：
    CSB-E13406B
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Bovine AMH ELISA Kit was designed for the quantitative measurement of Bovine AMH protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.5 ng/mL-200 ng/mL and the sensitivity is 0.5 ng/mL .

  • 别名：
    AMH ELISA Kit; Muellerian-inhibiting factor ELISA Kit; Anti-Muellerian hormone ELISA Kit; AMH ELISA Kit; Muellerian-inhibiting substance ELISA Kit; MIS ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Bos taurus (Bovine)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.5 ng/mL-200 ng/mL
  • 灵敏度：
    0.5 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<15%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine AMH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %95
    Range %90-101
    1:2Average %98
    Range %91-105
    1:4Average %92
    Range %88-97
    1:8Average %97
    Range %93-105
  • 回收率：
    The recovery of bovine AMH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9489-99
    EDTA plasma (n=4)9790-103
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2Average
    2000.134 0.139 0.137
    37.50.211 0.203 0.207
    6.250.495 0.485 0.490
    1.750.968 0.987 0.978
    0.51.146 1.154 1.150
    02.226 2.250 2.238
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    This glycoprotein, produced by the Sertoli cells of the testis, causes regression of the Muellerian duct. It is also able to inhibit the growth of tumors derived from tissues of Muellerian duct origin.
  • 基因功能参考文献：
    1. Anti-Mullerian hormone inhibits activation and growth of bovine ovarian follicles in vitro and is localized to growing follicles. PMID: 28333275
    2. This study showed that antral follicle counts (AFC) and AMH levels were repeatable when determined at an unknown stage of follicular growth and expected day of follicular wave emergence, but repeatability was greater for AMH than AFC. PMID: 28237349
    3. The effects of castration and other surgical intervention on the blood levels of anti-Muellerian hormone, inhibin A, gonadotropins, and gonadotropin receptors in bull calves are reported. PMID: 27693012
    4. Results from these studies indicate that AMH signaling plays a role in both regulating granulosa cell proliferation and preventing granulosa cells from 5- to 8-mm follicles from undergoing premature differentiation before follicle selection. PMID: 27268296
    5. These findings indicate the followings: AMH mRNA levels decrease in both dominant and secondary follicles during follicular deviation; granulosa cells from heathy follicles express more AMH mRNA compared to subordinate follicles undergoing atresia and FSH stimulates AMH and AMHR2 mRNA expression in granulosa cells of co-dominant follicles. PMID: 26815645
    6. Measurement of AMH concentration in the plasma of cows can help to predict their capacity for embryo production in response to gonadotrophin treatment. PMID: 22935154
    7. In first study to investigate the blood profile and immunohuistochemistry of anti-Mullerian hormone in bovine granulosa-theca cell tumors, the findings indicated that anti-Mullerian hormone is a novel biomarker for granulosa-theca cell tumors in cattle. PMID: 22052009
    8. Regulation of anti-Mullerian hormone production in the cow. PMID: 21076084
    9. Intrafollicular AMH was not a marker of cystic development in the cow, but low AMH concentrations in cysts were associated with luteinization. PMID: 18448844
    10. AMH expression is modulated by androgens in bovine granulosa cells from small follicles. PMID: 19714502

    显示更多

    收起更多

  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    TGF-beta family
  • 数据库链接：

    KEGG: bta:280718

    STRING: 9913.ENSBTAP00000019912

    UniGene: Bt.7050