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  4. Bovine Thyroid Stimulating Hormone(TSH) ELISA Kit

Bovine Thyroid Stimulating Hormone(TSH) ELISA Kit

  • 中文名称：
    牛促甲状腺素(TSH)酶联免疫试剂盒
  • 货号：
    CSB-E17548B
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Bovine TSH ELISA Kit was designed for the quantitative measurement of Bovine TSH protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.9 uIU/mL-60 uIU/mL and the sensitivity is 0.225 μIU/mL.

  • 别名：
    TSHB ELISA Kit; Thyrotropin subunit beta ELISA Kit; Thyroid-stimulating hormone subunit beta ELISA Kit; TSH-B ELISA Kit; TSH-beta ELISA Kit; Thyrotropin beta chain ELISA Kit
  • 缩写：
    TSH
  • Uniprot No.：
  • 种属：
    Bos taurus (Bovine)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.9 uIU/mL-60 uIU/mL
  • 灵敏度：
    0.225 μIU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine TSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %97
    Range %92-101
    1:2Average %88
    Range %84-92
    1:4Average %90
    Range %85-98
    1:8Average %92
    Range %88-96
  • 回收率：
    The recovery of bovine TSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10095-104
    EDTA plasma (n=4)8480-88
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    µIU/ml OD1OD2AverageCorrected
    601.815 1.731 1.773 1.626
    301.279 1.237 1.258 1.111
    150.934 0.927 0.931 0.784
    7.50.623 0.671 0.647 0.500
    3.750.408 0.379 0.394 0.247
    1.80.315 0.297 0.306 0.159
    0.90.201 0.195 0.198 0.051
    00.150 0.143 0.147
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Indispensable for the control of thyroid structure and metabolism.
  • 基因功能参考文献：
    1. Nitric oxide decreases TSH-induced FoxE1 expression, thus repressing the transcripcional activation of TPO gene. PMID: 26610751
    2. identification for the first time that ADAM10 is a TSHR cleavage enzyme and demonstration that TSH regulates its activation PMID: 18074395
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Glycoprotein hormones subunit beta family
  • 数据库链接：

    KEGG: bta:281552

    STRING: 9913.ENSBTAP00000008264

    UniGene: Bt.475