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  4. Bovine Prolactin/Luteotropic Hormone(PRL/LTH) ELISA Kit

Bovine Prolactin/Luteotropic Hormone(PRL/LTH) ELISA Kit

  • 中文名称：
    牛催乳素(PRL/LTH)酶联免疫试剂盒
  • 货号：
    CSB-E14002B
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Bovine PRL ELISA Kit was designed for the quantitative measurement of Bovine PRL protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 20 μIU/mL-8000 μIU/mL and the sensitivity is 20 μIU/mL.

  • 别名：
    PRL ELISA Kit; Prolactin ELISA Kit; PRL ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Bos taurus (Bovine)
  • 样本类型：
    serum, plasma
  • 检测范围：
    20 μIU/mL-8000 μIU/mL
  • 灵敏度：
    20 μIU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine PRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 95  
    Range % 91-100  
    1:2 Average % 95  
    Range % 86-104  
    1:4 Average % 90  
    Range % 85-95  
    1:8 Average % 96  
    Range % 92-108  
  • 回收率：
    The recovery of bovine PRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 91-102  
    EDTA plasma (n=4) 95 89-102  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μIU/ml OD1 OD2 Average    
    8000 0.361 0.369 0.365    
    1500 0.695 0.704 0.700 　  
    250 1.205 1.199 1.202    
    70 1.524 1.520 1.522    
    20 1.703 1.709 1.706    
    0 2.245 2.243 2.244    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Prolactin acts primarily on the mammary gland by promoting lactation.
  • 基因功能参考文献：
    1. Taken together, these results support novel functions of prolactin as a modulator of the innate immune response that do not involve the classical prolactin pathway. PMID: 26341952
    2. The presence and localization of prolactin receptor are consistent with expression data reported for other species, and the presence of PIP and prolactin in seminal fluid is consistent with data generated in humans. PMID: 25533929
    3. Functionally reciprocal mutations of the prolactin signaling pathway define hairy and slick cattle. PMID: 25519203
    4. The single nucleotide polymorphism rs42646708 of cattle XKR4 was significantly associated with serum prolactin concentrations and explained 2.45% of the phenotypic variation. PMID: 24666329
    5. Five mutations were identified in exonic region and eleven in associated intronic regions in PRL gene in 100 cattle from four Pakistani cattle breeds. PMID: 24065524
    6. this study identifies a biochemical mechanism for the regulation of SCFAs on bovine GH and PRL gene transcription in dairy cow anterior pituitary cells PMID: 24177567
    7. These data suggest that PRL protects brain endothelial cells against methamphetamine-induced toxicity PMID: 23988027
    8. Bovine prolactin improved the expression of human transferrin through such a possible mechanism that bovine prolactin activated STAT5a transcription expression. PMID: 22829284
    9. There was no significant difference between 1134 locus and milk performance traits of 5'-UTR of PRL gene PMID: 22207382
    10. Staphylococcus aureus infection inhibits nuclear factor kappa B activation mediated by prolactin in bovine mammary epithelial cells. PMID: 21843629
    11. Study of genetic variation in Yakutian cattle (Bos taurus L.) using the prolactin bPRL, growth hormone bGH, and transcription factor bPit-1 genes PMID: 20391788
    12. This is the second study reporting single nucleotide polymorphisms in the 5'-regulatory region of PRL gene, which interfere with milk production traits. PMID: 19714484
    13. evidence for a functional coupling between the PRL salt bridge and phosphorylation site indicates that either in vivo phosphorylation or specific mutations that destabilize the salt bridge impair biological activity. PMID: 12850287
    14. determination of the effects of exposure to different lengths of daylight during the dry period on circulating PRL and PRL receptor mRNA expression in lymphocytes and mammary tissue during the transition to lactation PMID: 15591374
    15. Expression of PRL and prolactin receptor (PRLR) mRNA is demonstrated for the first time in bovine corpus luteum throughout the luteal phase. PMID: 16435374
    16. The A-->G transition at position -1043 abolishes the recognition site for Hsp92II restriction endonuclease. PMID: 17929163
    17. Prolactin in ovarian follicular fluid stimulates endothelial cell proliferation. PMID: 19672107

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Somatotropin/prolactin family
  • 数据库链接：

    KEGG: bta:280901

    STRING: 9913.ENSBTAP00000020313

    UniGene: Bt.7197