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  4. Bovine Interleukin 12,IL-12 ELISA Kit

Bovine Interleukin 12,IL-12 ELISA Kit

  • 中文名称：
    牛白介素12(IL-12)酶联免疫试剂盒
  • 货号：
    CSB-E14010B
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Bovine IL12A ELISA Kit was designed for the quantitative measurement of Bovine IL12A protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL .

  • 别名：
    IL12AInterleukin-12 subunit alpha ELISA Kit; IL-12A ELISA Kit; Cytotoxic lymphocyte maturation factor 35 kDa subunit ELISA Kit; CLMF p35 ELISA Kit; IL-12 subunit p35 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Bos taurus (Bovine)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    6.25 pg/mL-400 pg/mL
  • 灵敏度：
    1.56 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %86
    Range %80-92
    1:2Average %98
    Range %91-102
    1:4Average %94
    Range %90-98
    1:8Average %94
    Range %86-99
  • 回收率：
    The recovery of bovine IL-12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8984-92
    EDTA plasma (n=4)9795-101
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    4002.758 2.628 2.693 2.531
    2002.184 2.084 2.134 1.972
    1001.612 1.562 1.587 1.425
    501.203 1.173 1.188 1.026
    250.652 0.642 0.647 0.485
    12.50.384 0.374 0.379 0.217
    6.250.258 0.253 0.256 0.094
    00.163 0.161 0.162 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.
  • 基因功能参考文献：
    1. The results showed that the expression of TNF-alpha, iNOS, and IL-6 in alveolar macrophages was up-regulated by stimulation with the recombinant Mce4A protein of M. bovis; in contrast, expression of IL-12 was unaffected.[IL-6, IL-12] PMID: 17530193
    2. Lipopolysaccharide stimulation up-regulates the secretion of cytokines, including interleukin-12, by bovine polymorphonuclear neutrophil leukocytes. PMID: 17727806
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    IL-6 superfamily
  • 数据库链接：

    KEGG: bta:281856

    STRING: 9913.ENSBTAP00000020153

    UniGene: Bt.524