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Bovine Interleukin 10,IL-10 ELISA Kit

  • 中文名称：
    牛白介素10，IL-10酶联免疫试剂盒
  • 货号：
    CSB-E12917B
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Bovine IL10 ELISA Kit was designed for the quantitative measurement of Bovine IL10 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 5 pg/mL-1000 pg/mL and the sensitivity is 2.5 pg/mL.

  • 别名：
    IL10Interleukin-10 ELISA Kit; IL-10 ELISA Kit; Cytokine synthesis inhibitory factor ELISA Kit; CSIF ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Bos taurus (Bovine)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    5 pg/mL-1000 pg/mL
  • 灵敏度：
    2.5 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 94  
    Range % 89-98  
    1:2 Average % 95  
    Range % 91-98  
    1:4 Average % 96  
    Range % 87-102  
    1:8 Average % 97  
    Range % 91-104  
  • 回收率：
    The recovery of bovine IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 97 95-100  
    EDTA plasma (n=4) 96 92-103  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    1000 0.098 0.095 0.097    
    250 0.301 0.285 0.293    
    80 0.487 0.458 0.473    
    20 0.972 0.975 0.974    
    5 1.364 1.316 1.340    
    0 2.128 2.211 2.170    
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 功能：
    Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation. In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling.
  • 基因功能参考文献：
    1. Studied expression of Interleukin 10 (IL-10) in blood and milk samples of 25 healthy and 25 mastitic cows. Found IL-10 expression to be significantly higher in the blood and milk samples of mastitic cows compared to the healthy ones. PMID: 28267404
    2. This study seems to indicate that PGE2 in cattle does not produce an anti-inflammatory effect by increasing the synthesis of IL-10. PMID: 25286662
    3. IL-10 mRNA expression increased on day 8 in the mononuclear leukocytes of pregnant cows. In a cell culture experiment, interferon-tau stimulated stimulated IL-10 mRNA expression in mononuclear leukocytes. PMID: 22052007
    4. Bovine IL-10 potently inhibits the activation of human myeloid cells in response to toll like receptor activation. PMID: 21464967
    5. Levels in milk are altered in the presence of bacteria. PMID: 15372997
    6. Monocytes obtained from cows with subclinical infection with MAP had upregulated expression of IL-10 and SOCS-3, which may have attenuated the capacity of mononuclear phagocytes to initiate inflammatory and adaptive immune responses. PMID: 16111147

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    IL-10 family
  • 数据库链接：

    KEGG: bta:281246

    STRING: 9913.ENSBTAP00000008793

    UniGene: Bt.4723