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  4. Bovine Interleukin 1,IL-1 ELISA Kit

Bovine Interleukin 1,IL-1 ELISA Kit

  • 中文名称：
    牛白介素1(IL-1)酶联免疫试剂盒
  • 货号：
    CSB-E12019B
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Bovine IL1A ELISA Kit was designed for the quantitative measurement of Bovine IL1A protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 250 pg/mL-4000 pg/mL and the sensitivity is 250 pg/mL.

  • 别名：
    IL1A ELISA Kit; Interleukin-1 alpha ELISA Kit; IL-1 alpha ELISA Kit
  • 缩写：
    IL-1
  • Uniprot No.：
  • 种属：
    Bos taurus (Bovine)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    250 pg/mL-4000 pg/mL
  • 灵敏度：
    250 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 90  
    Range % 85-94  
    1:2 Average % 92  
    Range % 87-97  
    1:4 Average % 84  
    Range % 80-88  
    1:8 Average % 98  
    Range % 90-102  
  • 回收率：
    The recovery of bovine IL-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 90-98  
    EDTA plasma (n=4) 89 83-96  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    4000 0.147 0.141 0.144    
    2000 0.296 0.279 0.288    
    1000 0.431 0.467 0.449    
    500 0.782 0.823 0.803    
    250 1.862 1.826 1.844    
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 功能：
    Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
  • 基因功能参考文献：
    1. These findings suggest that the elevated levels of IL-1alpha found in the osteoarthritis environment heighten fibroblast-like synoviocytes sensitivity to fluid shear by altering both intercellular communication and individual cell sensitivity, which could affect downstream functions and contribute to progression of the disease state PMID: 28716465
    2. Protein kinase R plays a pivotal role in oncostatin M and interleukin-1 signalling in bovine articular cartilage chondrocytes. PMID: 22287113
    3. Testicular IL-1 alpha and IL-1 beta concentrations were highest in the early post-natal period; however, IL-1 bioactivity and IL-6 concentrations were greatest in the immediate pre-pubertal period. PMID: 19144032
    4. Genes for IL-1alpha and IL-1beta are expressed and a functional IL-1R is present in bovine corpora lutea throughout luteal phase. IL-1alpha and IL-1beta may have different roles as regulating PGF(2alpha) and PGE(2) production during luteal phase. PMID: 15253926
    5. non-metalloproteinase mechanisms participate in IL-1-induced matrix degradation and loss of tissue material properties PMID: 17174540
    6. These results suggest that IL-1alpha and IL-1beta are produced by the stromal cells, that IL-1beta is produced by the epithelial cells, and that IL-1alpha is a more potent stimulator of prostaglandin and plasminogen activator in bovine endometrial cells. PMID: 18930361

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  • 亚细胞定位：
    Cytoplasm. Secreted.
  • 蛋白家族：
    IL-1 family
  • 数据库链接：

    KEGG: bta:281250

    STRING: 9913.ENSBTAP00000013665

    UniGene: Bt.191