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  4. Bovine Inhibin beta A chain(INHBA) ELISA kit

Bovine Inhibin beta A chain(INHBA) ELISA kit

  • 中文名称：
    牛抑制素-βA链(INHBA)酶联免疫试剂盒
  • 货号：
    CSB-EL011719BO
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Bovine INHBA ELISA Kit was designed for the quantitative measurement of Bovine INHBA protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 15 pg/mL-600 pg/mL and the sensitivity is 15 pg/mL.

  • 别名：
    INHBAInhibin beta A chain ELISA Kit; Activin beta-A chain ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Bos taurus (Bovine)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15 pg/mL-600 pg/mL
  • 灵敏度：
    15 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine INHBA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 95  
    Range % 91-98  
    1:2 Average % 94  
    Range % 86-103  
    1:4 Average % 92  
    Range % 89-98  
    1:8 Average % 96  
    Range % 91-102  
  • 回收率：
    The recovery of bovine INHBA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 92-100  
    EDTA plasma (n=4) 96 91-103  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average 　  
    600 0.161 0.159 0.160 　  
    300 0.371 0.353 0.362 　  
    138 0.586 0.555 0.571 　  
    45 0.867 0.874 0.871 　  
    15 1.489 1.492 1.491 　  
    0 2.368 2.441 2.405 　  
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.
  • 基因功能参考文献：
    1. Results confirm the prodevelopmental actions of activin A and indicate that CTGF may also function as an embryokine by regulating the number of ICM cells in the blastocyst and altering gene expression. Low concentrations of HGF were inhibitory to development. PMID: 27185102
    2. The expression of the components of the activin-inhibin-follistatin system is altered in bovine cystic ovarian disease. PMID: 25001504
    3. Results support a physiological role for granulosa-derived inhibin as an antagonist of bone morphogenetic protein action on thecal androgen synthesis. PMID: 20739376
    4. activin AB is a lignd for activin type IB receptor ALK7 and is responsible for activin-mediated secretion of insulin from pancreatic beta cell line, MIN6. PMID: 15196700
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    TGF-beta family
  • 数据库链接：