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  4. Bovine Heat Shock Protein 72 (HSP-72) ELISA Kit

Bovine Heat Shock Protein 72 (HSP-72) ELISA Kit

  • 中文名称：
    牛热休克蛋白72(HSP-72)酶联免疫试剂盒
  • 货号：
    CSB-E15831B
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Bovine HSPA2 ELISA Kit was designed for the quantitative measurement of Bovine HSPA2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL .

  • 别名：
    HSPA2 ELISA Kit; HSP70-3 ELISA Kit; HSPA3 ELISA Kit; Heat shock-related 70 kDa protein 2 ELISA Kit; Heat shock 70 kDa protein 3 ELISA Kit; HSP70.3 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Bos taurus (Bovine)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine HSP-72 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %95
    Range %87-98
    1:2Average %97
    Range %92-105
    1:4Average %98
    Range %94-108
    1:8Average %86
    Range %82-92
  • 回收率：
    The recovery of bovine HSP-72 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9488-98
    EDTA plasma (n=4)9287-96
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    202.532 2.432 2.482 2.349
    101.974 1.874 1.924 1.791
    51.271 1.171 1.221 1.088
    2.50.805 0.785 0.795 0.662
    1.250.460 0.450 0.455 0.322
    0.6250.338 0.328 0.333 0.200
    0.3120.228 0.218 0.223 0.090
    00.134 0.132 0.133 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. In the ATP-bound form, it has a low affinity for substrate proteins. However, upon hydrolysis of the ATP to ADP, it undergoes a conformational change that increases its affinity for substrate proteins. It goes through repeated cycles of ATP hydrolysis and nucleotide exchange, which permits cycles of substrate binding and release. Plays a role in spermatogenesis. In association with SHCBP1L may participate in the maintenance of spindle integrity during meiosis in male germ cells.
  • 亚细胞定位：
    Cytoplasm, cytoskeleton, spindle.
  • 蛋白家族：
    Heat shock protein 70 family
  • 数据库链接：

    KEGG: bta:281827

    UniGene: Bt.19759