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Bovine Deoxyribonuclease-1(DNASE1) ELISA kit

  • 中文名称：
    牛脱氧核糖核酸酶1(DNASE1)酶联免疫试剂盒
  • 货号：
    CSB-EL007049BO
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Bovine DNASE1 ELISA Kit was designed for the quantitative measurement of Bovine DNASE1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 pg/mL-800 pg/mL and the sensitivity is 3.1 pg/mL.

  • 别名：
    DNASE1 ELISA Kit; DNL1Deoxyribonuclease-1 ELISA Kit; EC 3.1.21.1 ELISA Kit; Deoxyribonuclease I ELISA Kit; DNase I ELISA Kit
  • 缩写：
    DNASE1
  • Uniprot No.：
  • 种属：
    Bos taurus (Bovine)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    12.5 pg/mL-800 pg/mL
  • 灵敏度：
    3.1 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Epigenetics and Nuclear Signaling
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine DNASE1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:20 Average % 85
    Range % 80-89
    1:40 Average % 94
    Range % 90-98
    1:80 Average % 103
    Range % 99-109
    1:160 Average % 97
    Range % 92-101
  • 回收率：
    The recovery of bovine DNASE1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 88 84-92
    EDTA plasma (n=4) 93 89-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    800 2.399 2.416 2.408 2.273
    400 2.213 2.195 2.204 2.069
    200 1.813 1.741 1.777 1.642
    100 1.204 1.227 1.216 1.081
    50 0.683 0.713 0.698 0.563
    25 0.400 0.392 0.396 0.261
    12.5 0.292 0.284 0.288 0.153
    0 0.131 0.138 0.135 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Serum endocuclease secreted into body fluids by a wide variety of exocrine and endocrine organs. Expressed by non-hematopoietic tissues and preferentially cleaves protein-free DNA. Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization. Together with DNASE1L3, plays a key role in degrading neutrophil extracellular traps (NETs). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation. Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation.
  • 基因功能参考文献：
    1. ascorbic acid has the ability to inhibit DNase I, one of the main endonucleases involved in DNA fragmentation during apoptosis. PMID: 29551685
    2. the practical capability of this assay system was successfully demonstrated by its use to determine DNase I activity in bovine urine. PMID: 27570054
    3. Specificity in DNA-DNase I interaction is mediated by DNA flexibility, which influences the induced-fit transitions required to form productive complexes. PMID: 19850052
    4. results indicated that the backbone hydrogen bonding in the antiparallel beta-sheet formed by its N- and C-termini, rather than the side-chain interactions, is crucial for the correct protein folding PMID: 15311926
  • 亚细胞定位：
    Secreted. Zymogen granule. Nucleus envelope.
  • 蛋白家族：
    DNase I family
  • 数据库链接：