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  4. Bovine Apolipoprotein A-I(APOA1) ELISA kit

Bovine Apolipoprotein A-I(APOA1) ELISA kit

  • 中文名称：
    牛载脂蛋白A1(APOA1)酶联免疫试剂盒
  • 货号：
    CSB-EL001913BO
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Bovine APOA1 ELISA Kit was designed for the quantitative measurement of Bovine APOA1 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 78 ng/mL-5000 ng/mL and the sensitivity is 19.5 ng/mL .

  • 别名：
    APOA1Apolipoprotein A-I ELISA kit; Apo-AI ELISA kit; ApoA-I ELISA kit; Apolipoprotein A1) [Cleaved into: Proapolipoprotein A-I ELISA kit; ProapoA-I); Truncated apolipoprotein A-I] ELISA kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Bos taurus (Bovine)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    78 ng/mL-5000 ng/mL
  • 灵敏度：
    19.5 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine APOA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1000Average %90
    Range %87-94
    1:2000Average %96
    Range %91-100
    1:4000Average %93
    Range %87-97
    1:8000Average %100
    Range %96-103
  • 回收率：
    The recovery of bovine APOA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8482-87
    EDTA plasma (n=4)8985-96
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2Average
    50000.140 0.143 0.142
    25000.285 0.291 0.288
    12500.482 0.503 0.493
    6250.814 0.821 0.818
    3121.253 1.268 1.261
    1561.695 1.709 1.702
    782.040 2.134 2.087
    02.436 2.458 2.447
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
  • 基因功能参考文献：
    1. the model of a two-step process for the transendothelial transport of apoA-I in which apoA-I is initially lipidated by ABCA1 and then further processed by ABCA1-independent mechanisms. PMID: 21209084
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Apolipoprotein A1/A4/E family
  • 组织特异性：
    Major protein of plasma HDL, also found in chylomicrons.
  • 数据库链接：