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  4. Bovine Alpha-2-macroglobulin(A2M) ELISA kit

Bovine Alpha-2-macroglobulin(A2M) ELISA kit

  • 中文名称：
    牛α2巨球蛋白(A2M)酶联免疫试剂盒
  • 货号：
    CSB-EL001004BO
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Bovine A2M ELISA Kit was designed for the quantitative measurement of Bovine A2M protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.312 μg/mL- 20 μg/mL and the sensitivity is 0.078 μg/mL.

  • 别名：
    A2MAlpha-2-macroglobulin ELISA kit; Alpha-2-M ELISA kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Bos taurus (Bovine)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.312 μg/mL- 20 μg/mL
  • 灵敏度：
    0.078 μg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine A2M in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:100Average %98
    Range %90-103
    1:200Average %90
    Range %82-94
    1:400Average %101
    Range %95-106
    1:800Average %108
    Range %101-112
  • 回收率：
    The recovery of bovine A2M spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8882-91
    EDTA plasma (n=4)9387-99
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/mlOD1OD2Average
    200.127 0.131 0.129
    100.248 0.233 0.241
    50.490 0.523 0.507
    2.50.909 0.911 0.910
    1.251.439 1.464 1.452
    0.6251.853 1.789 1.821
    0.3122.460 2.400 2.430
    02.795 2.702 2.749
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
  • 基因功能参考文献：
    1. Mutations in the promoter region and the 3'-untranslated region of the A2M gene are involved in the mastitis susceptibility in dairy cows. PMID: 25237709
    2. We report here for the first time the direct isolation of Spp24 covalently bound to alpha2-macroglobulins as a core complex (Spp24-alpha2M) from bovine calf serum. PMID: 22949401
    3. Exon 29 c.3535A>T in the alpha-2-macroglobulin gene causing aberrant splice variants is associated with mastitis. PMID: 22923050
    4. These results suggest the possible involvement of cathepsin E in disruption of the structural and functional integrity of alpha 2-macroglobulin in the endolysosome system. PMID: 12631277
    5. Alpha2-macroglobulin is a substrate and an endogenous inhibitor for ADAMTS-4 and ADAMTS-5 PMID: 14715656
    6. Amounts of alpha(2)-M receptor in granulosa cells were strongly inversely associated with concentrations of estradiol in dominant and subordinate follicles. PMID: 15001551

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Protease inhibitor I39 (alpha-2-macroglobulin) family
  • 组织特异性：
    Plasma.
  • 数据库链接：