Hrh3

Overlay Peak curve showing SH-SY5Y cells surface stained with CSB-RA783626A0HU (red line) at 1:50. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Western Blot
Positive WB detected in: A549 whole cell lysate
All lanes: HRH3 antibody at 2.5µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 49, 50, 41, 48, 37, 46 kDa
Observed band size: 49 kDa

Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA897568LA01HU at dilution of 1:100

Immunofluorescent analysis of A549 cells using CSB-PA897568LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)