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  4. Mouse N-terminal pro-brain natriuretic peptide,NT-proBNP ELISA KIT

Mouse N-terminal pro-brain natriuretic peptide,NT-proBNP ELISA KIT

  • 中文名称：
    小鼠N端前脑钠素(NT-proBNP)酶联免疫试剂盒
  • 货号：
    CSB-E05153m
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Mouse NT-proBNP ELISA Kit was designed for the quantitative measurement of Mouse NT-proBNP protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

  • 缩写：
    NT-proBNP
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse NT-proBNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 90
    Range % 85-96
    1:2 Average % 96
    Range % 91-105
    1:4 Average % 89
    Range % 85-99
    1:8 Average % 93
    Range % 83-98
  • 回收率：
    The recovery of mouse NT-proBNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 95 87-105
    EDTA plasma (n=4) 95 91-99
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    10 2.235 2.199 2.217 2.090
    5 1.884 1.749 1.817 1.690
    2.5 1.462 1.375 1.419 1.292
    1.25 0.978 1.035 1.007 0.880
    0.625 0.552 0.549 0.551 0.424
    0.312 0.289 0.284 0.287 0.160
    0.156 0.203 0.211 0.207 0.080
    0 0.128 0.125 0.127 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:


1. Are the antibodies in the kit monoclonal or polyclonal ?
2. If the antibodies are polyclonal, please kindly let us know the HOST, antigen, and the purification method
(Affinity chromatography or protein A (G) etc.)
3. what is stop solution ? 1M Hydrochloric Acid ?
4. If the kit includes vaculovirus or other virus expressions,
please kindly let us know which items in the kit include it.
5. Please kindly advise the source of Standard. synthesis ? or Recombinant ? or Extraction ? or other ?
6. If it is recombinant, what is the host.
7. Please let us know whether the antibody and the standard include any preservative or not.
ex. thimerosal or sodium azide or 2-mercaptoethanol etc.

A:
Thanks for your inquiry.
Capture antibody is goat polyclonal antibody and detection antibody is goat polyclonal antibody, affinity chromatography.
Stop solution is 4N sulfuric acid.
Standard is 293 cell-derived recombinant protein）.
The antibody and the standard contain Preservative：proclin 300(1:3000).
The kit doesn't contain thimerosal or sodium azide or 2-mercaptoethanol etc.
Pls let me know if you have any further questions. Thank you.