Canine Creatine Kinase MB isoenzyme (CK-MB)ELISA Kit

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产品详情

产品描述：

This Dog CK-MB ELISA Kit was designed for the quantitative measurement of Dog CK-MB protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 1.56 μIU/mL-100 μIU/mL and the sensitivity is 0.78 μIU/mL.
缩写：

CK-MB
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：

serum, plasma
检测范围：

1.56 μIU/mL-100 μIU/mL
灵敏度：

0.78 μIU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine CK-MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	84
	Range %	80-88
1:200	Average %	98
	Range %	94-102
1:400	Average %	90
	Range %	86-94
1:800	Average %	92
	Range %	88-96

回收率：

The recovery of canine CK-MB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	95-107
EDTA plasma (n=4)	89	85-93

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.