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  4. Recombinant Mouse Omega-3 fatty acid receptor 1 (O3far1)

Recombinant Mouse Omega-3 fatty acid receptor 1 (O3far1)

  • 中文名称：
    小鼠Ffar4重组蛋白
  • 货号：
    CSB-CF749243MO
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
  • Uniprot No.：
  • 别名：
    Ffar4; Gpr120; O3far1; Free fatty acid receptor 4; G-protein coupled receptor 120; G-protein coupled receptor GT01; Omega-3 fatty acid receptor 1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-361
  • 氨基酸序列
    MSPECAQTTGPGPSHTLDQVNRTHFPFFSDVKGDHRLVLSVVETTVLGLIFVVSLLGNVC ALVLVARRRRRGATASLVLNLFCADLLFTSAIPLVLVVRWTEAWLLGPVVCHLLFYVMTM SGSVTILTLAAVSLERMVCIVRLRRGLSGPGRRTQAALLAFIWGYSALAALPLCILFRVV PQRLPGGDQEIPICTLDWPNRIGEISWDVFFVTLNFLVPGLVIVISYSKILQITKASRKR LTLSLAYSESHQIRVSQQDYRLFRTLFLLMVSFFIMWSPIIITILLILIQNFRQDLVIWP SLFFWVVAFTFANSALNPILYNMSLFRNEWRKIFCCFFFPEKGAIFTDTSVRRNDLSVIS S
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    G-protein-coupled receptor for long-chain fatty acids (LCFAs) with a major role in adipogenesis, energy metabolism and inflammation. Signals via G-protein and beta-arrestin pathways. LCFAs sensing initiates activation of phosphoinositidase C-linked G proteins GNAQ and GNA11 (G(q)/G(11)), inducing a variety of cellular responses via second messenger pathways such as intracellular calcium mobilization, modulation of cyclic adenosine monophosphate (cAMP) production, and mitogen-activated protein kinases (MAPKs). After LCFAs binding, associates with beta-arrestin ARRB2 that acts as an adapter protein coupling the receptor to specific downstream signaling pathways, as well as mediating receptor endocytosis. In response to dietary fats, plays an important role in the regulation of adipocyte proliferation and differentiation. Acts as a receptor for omega-3 polyunsaturated fatty acids (PUFAs) at primary cilium of perivascular preadipocytes, initiating an adipogenic program via cAMP and CTCF-dependent chromatin remodeling that ultimately results in transcriptional activation of adipogenic genes and cell cycle entry. Induces differentiation of brown and beige adipocytes probably via autocrine and endocrine functions of FGF21 hormone. Contributes to the thermogenic activation of brown adipose tissue and the browning of white adipose tissue. Activates brown adipocytes by initiating intracellular calcium signaling leading to mitochondrial depolarization and fission, and overall increased mitochondrial respiration. Consequently stimulates fatty acid uptake and oxidation in mitochondria together with UCP1-mediated thermogenic respiration, eventually reducing fat mass. Regulates bi-potential differentiation of bone marrow mesenchymal stem cells toward osteoblasts or adipocytes likely by up-regulating distinct integrins. In response to dietary fats regulates hormone secretion and appetite. Stimulates GIP and GLP1 secretion from enteroendocrine cells as well as GCG secretion in pancreatic alpha cells, thereby playing a role in the regulation of blood glucose levels. Negatively regulates glucose-induced SST secretion in pancreatic delta cells. Mediates LCFAs inhibition of GHRL secretion, an appetite-controlling hormone. In taste buds, contributes to sensing of dietary fatty acids by the gustatory system. During the inflammatory response, promotes anti-inflammatory M2 macrophage differentiation in adipose tissue. Mediates the anti-inflammatory effects of omega-3 PUFAs via inhibition of NLRP3 inflammasome activation. In this pathway, interacts with adapter protein ARRB2 and inhibits the priming step triggered by Toll-like receptors (TLRs) at the level of TAK1 and TAB1. Further inhibits the activation step when ARRB2 directly associates with NLRP3, leading to inhibition of proinflammatory cytokine release. Mediates LCFAs anti-apoptotic effects.
  • 基因功能参考文献：
    1. FFAR4 is differentially expressed and correlated to cytokine expressions in peritoneal macrophages and alveolar macrophages from BALB/c mice. PMID: 29595453
    2. Ovarian hormones may directly regulate GPR120 expression in the reproductive cycle at the pituitary level. PMID: 28824022
    3. this is the first animal study to provide evidence that FFA4 may mediate the protective roles of n-3 fatty acids on lean mass percentage as well as fat mass percentage. PMID: 28900676
    4. Data suggest that omega-3 fatty acids, common dietary lipids, participate in immunomodulation; here, EPA (eicosapentaenoic acid) activates macrophage RAW264.7 cells through GPR120-mediated Raf-ERK1/2-IKKbeta-NFkappaB p65 signaling pathways. PMID: 28841192
    5. GPR120 suppresses adipose tissue lipolysis and synergizes with GPR40 in antidiabetic efficacy PMID: 28583918
    6. Docosahexaenoic acid activates GPR120 to prevent experimental colitis in IL-10 deficient mice. PMID: 28039475
    7. GPR120 (and GPR40) act in concert in the hypothalamus to reduce energy efficiency and regulate the inflammation associated with obesity. PMID: 28446241
    8. Acute reductions in food intake and food reward suggest that GPR120 could mediate the effects of central omega-3 polyunsaturated fatty acids to inhibit appetite. PMID: 26888796
    9. These data reveal important structure-function and signaling differences between the two FFA4 isoforms, and for the first time link FFA4 to modulation of ROS in macrophages. PMID: 28943238
    10. Results provide evidence that GPR120 promotes adipogenesis by increasing PPARgamma expression via [Ca(2+)]i and ERK1/2 signal pathway in 3T3-L1 adipocytes. PMID: 27302893
    11. GPR120-induced incretin glucse-dependent insulinotropic polypeptide secretion is indirectly mediated by cholecystokinin. PMID: 28324023
    12. Leukocyte GPR120/FFAR4 WT or KO mice in the LDL receptor KO background were generated by bone marrow transplantation.leukocyte GPR120 expression has minimal effects on dietary PUFA-induced plasma lipid/lipoprotein reduction and atheroprotection, and there is no distinction between n-3 versus n-6 PUFAs in activating anti-inflammatory effects of leukocyte GPR120/FFAR4 in vivo PMID: 27811230
    13. Collectively, these findings showed that SA suppressed mammary gland development of pubertal mice, which was coincident with the SA-inhibited HC11 proliferation, and was associated with inhibition of PI3K/Akt signaling pathway through activation of GPR120. PMID: 28712865
    14. omega-3 fatty acids-induced proliferation of bronchiole epithelial cells through FFA4 is responsible for Omacor-induced accelerated recovery from airway injury. PMID: 28314803
    15. GPR120 silencing in adipocytes inhibited the expression of PPARgamma and miR-143, whereas GPR120 overexpression led to increased expressions of PPARgamma and miR-143. PMID: 28495174
    16. GPR120 expression in beta-cells and GPR120-mediated insulinotropic effects are altered in obesity and diabetic states in distinct ways, and these alterations may be mediated by PPARgamma. PMID: 27980130
    17. Data show that agonizing G protein-coupled receptor 120 (GPR120) differentially regulates the pro-inflammatory adipocytokines. PMID: 28263744
    18. FFA4 is a dual-acting factor that increases osteoblastic bone formation and decreases osteoclastic bone resorption PMID: 27145004
    19. work identifies a novel function of Ffar4 in modulating brown adipogenesis partly through a mechanism involving cAMP activation and up-regulation of miR-30b and miR-378. PMID: 27489163
    20. GPR120 is a negative modulator of osteoclast development. PMID: 26280807
    21. demonstrated a GPR120-mediated novel anti-inflammatory pathway in specific intestinal epithelial cell types that could be of therapeutic relevance to intestinal inflammatory disorders PMID: 26791484
    22. Data suggest phosphorylation of Ffar4 occurs at 3 serine and 2 threonine residues clustered in 2 separable regions of C-terminal tail; recruitment of arrestin 3, receptor internalization, and activation of Akt are regulated by Ffar4 phosphorylation. PMID: 26873857
    23. GPR120-mediated cellular signalling determines the bi-potential differentiation of BMMSCs in a dose-dependent manner. PMID: 26365922
    24. GPR 40/120 double-knockout mice are impaired in post-oral fat sensing. PMID: 25911263
    25. co-transfection of GPR120 enhanced eicosapentaenoic acid-induced PPARgamma binding to PPAR-response element in VEGF-A promoter region. PMID: 25697344
    26. results indicated that the beneficial metabolic role of DHA was attributed to its ability to regulate glucose via the GPR120-mediated AMPK pathway in the skeletal muscles PMID: 26134561
    27. The GPR120 in mouse pituitary gonadotropes is upregulated by fasting and that it may play a role in controlling gonadotropin secretion. PMID: 25112963
    28. Results suggest that GPR120 is not indispensable for the improved metabolic profile associated with intake of a diet enriched in n-3 polyunsaturated fatty acids. PMID: 25541716
    29. GPR120 is expressed abundantly in K cells of the upper small intestine and plays a critical role in lipid-induced GIP secretion. PMID: 25535828
    30. The results imply that GPR120 is selectively present within the delta cells of murine islets and that it regulates somatostatin secretion. PMID: 24663807
    31. GPR120 is a nutrient sensor that is activated endogenously by both saturated and unsaturated long chain fatty acids. PMID: 24742677
    32. CD36 and GPR120 have nonoverlapping roles in taste bud cell signaling during orogustatory perception of dietary lipids; these are differentially regulated by obesity. PMID: 24412488
    33. GPR40 and GPR120, two fatty acid responsive G-protein-coupled receptors (GPCRs), are expressed in taste bud cells, and knockout mice lacking either of those receptors have blunted taste nerve responses PMID: 23831422
    34. results suggest that the decrease of ghrelin secretion after feeding is induced partially by long-chain fatty acids that act directly on gastric G-protein coupled receptor 120(GPR120)-expressing ghrelin cells PMID: 24222669
    35. GPR40 and GPR120 fatty acid sensors are critical for postoral but not oral mediation of fat preferences in the mouse. PMID: 24154510
    36. GPR120-deficient mice fed a high-fat diet develop obesity, glucose intolerance and fatty liver with decreased adipocyte differentiation and lipogenesis and enhanced hepatic lipogenesis PMID: 22343897
    37. In contrast to GPR120, CD36 appears to be a food-sensitive lipid sensor in the gustatory circumvallate papillae PMID: 21901153
    38. GPR120 is a functional omega-3 fatty acid receptor/sensor and mediates potent insulin sensitizing and antidiabetic effects in vivo by repressing macrophage-induced tissue inflammation. PMID: 20813258
    39. GPR120 is expressed in the taste buds, mainly in type ii cells. GPR120 knockout mice show a diminished preference for linoleic acid and oleic acid. PMID: 20573884
    40. Regulates adipogenic processes such as adipocyte development and differentiation. PMID: 17250804
    41. long-chain free fatty acids induce cholecystokinin secretion through GPR120-coupled Ca2+ signaling. PMID: 17972064
    42. GPR120 is mainly expressed in the type II taste cells and might function as a sensor for dietary fat. PMID: 19071193
    43. Immunoreactivity for GPR120 was abundant in the mouse large intestine, lung, and adipose tissue. Expression of GPR120 protein was up-regulated during the adipogenic differentiation of 3T3-L1 cells. PMID: 19145429

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein. Endosome membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein. Cell projection, cilium membrane; Multi-pass membrane protein.
  • 蛋白家族：
    G-protein coupled receptor 1 family
  • 组织特异性：
    Highly expressed in brown and white adipose tissue. Expressed in perivascular ciliated preadipocytes (at protein level). Expressed in the taste buds of the circumvallate and fungiform papillae, mainly in type II cells (at protein level). Abundant expressi
  • 数据库链接：

    KEGG: mmu:107221

    STRING: 10090.ENSMUSP00000063660

    UniGene: Mm.35288