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  4. Recombinant Human Aquaporin-2 (AQP2)

Recombinant Human Aquaporin-2 (AQP2)

  • 中文名称：
    Recombinant Human Aquaporin-2 (AQP2)
  • 货号：
    CSB-CF001962HU
  • 规格：
    ￥9720
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 生物活性：
    Not Test
  • 基因名：
    AQP2
  • Uniprot No.：
  • 别名：
    AQP-2;ADH water channel;Aquaporin-CD;AQP-CD;Collecting duct water channel protein;WCH-CD;Water channel protein for renal collecting duct
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length
  • 来源：
    in vitro E.coli expression system
  • 分子量：
    30.3 kDa
  • 表达区域：
    1-271aa
  • 氨基酸序列
    MWELRSIAFSRAVFAEFLATLLFVFFGLGSALNWPQALPSVLQIAMAFGLGIGTLVQALGHISGAHINPAVTVACLVGCHVSVLRAAFYVAAQLLGAVAGAALLHEITPADIRGDLAVNALSNSTTAGQAVTVELFLTLQLVLCIFASTDERRGENPGTPALSIGFSVALGHLLGIHYTGCSMNPARSLAPAVVTGKFDDHWVFWIGPLVGAILGSLLYNYVLFPPAKSLSERLAVLKGLEPDTDWEEREVRRRQSVELHSPQSLPRGTKA
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Plays an essential role in renal water homeostasis.
  • 基因功能参考文献：
    1. Structural basis for mutations in human AQP2 associated with nephrogenic diabetes insipidus has been described. PMID: 29799470
    2. Morning urinary AQP2/Creatinine ratio was significantly lower in patients with nocturnal polyuria than in age-matched healthy controls. PMID: 29316008
    3. Here we provide an updated overview of the genetic defects causing NDI, the most recent strategies under investigation for rescuing the activity of mutated AVPR2 or AQP2, or for bypassing defective AVPR2 signaling and restoring AQP2 plasma membrane expression. PMID: 29125546
    4. Results indicate the functional recovery of recessive aquaporin 2 (AQP2) mutants through heteromerization. PMID: 27641679
    5. AQP2 is associated with the pathogenesis of femalestress urinary incontinence. PMID: 28713996
    6. Pretreatment with WRT also decreased the hypertonic stressinduced expression of AQP2, as with KT5720, a protein kinase A inhibitor. These results provided evidence of the beneficial effect of the traditional formula WRT in regulating water balance in hypertonic stress of the renal collecting ducts. PMID: 28447712
    7. E3 ligases most likely to interact with AQP2. PMID: 27199454
    8. The present study provides detailed insights into the transport properties of AQP2 with the use of microsecond-scale molecular dynamics simulations, and explains how these channels conduct water molecules while at the same time excluding other molecules. PMID: 27793629
    9. It is a predictor for the development of renal insufficiency and death of patients with liver cirrhosis. PMID: 28092112
    10. Data suggest that AQP2 binds LIP5 in a AQP2-phosphorylation-dependent manner; phospho-mimicking mutations and phosphorylation reduce thermal stability of AQP2; AQP2 phosphorylation allosterically controls its interaction with LIP5. [AQP2 = aquaporin 2; LIP5 = LYST-interacting protein 5; LYST = lysosomal trafficking regulator protein] PMID: 28710278
    11. The genetic variant rs426496 in AQP2; rs591810 in AQP3 and rs1805127, rs1805128, and rs17173510, in KCNE1 were found in patients with Meniere's disease PMID: 27509294
    12. aquaporin-2 regulates serine/threonine phosphatases in renal collecting duct PMID: 27784696
    13. An overview ofAQP2 mutations in genetic forms of nephrogenic diabetes insipidus (review) PMID: 27156763
    14. Impaired endometrial receptivity in patients who underwent controlled ovarian stimulation is correlated with a decreased expression of AQP2. PMID: 25232826
    15. Pretreatment with alkali (0.4 N NaOH) to disrupt exosome membranes allowed consistent ELISA measurements of urinary AQP2. PMID: 26463736
    16. Findings indicate that SIRT1 increases AQP2 expression in TNF-alpha-induced IMCD cells via the NF-kappaB-dependent signalling pathway, which might provide novel insight to understanding the renoprotective effects of SIRT1 in kidney diseases. PMID: 27980322
    17. AQP2 polymorphisms (rs461872, rs7305534) were correlated with gastrointestinal toxicity of platinum-based chemotherapy in lung cancer patients PMID: 26358256
    18. report a novel mutation of the AQP2 gene and highlight an important role of genetic testing for definite diagnosis PMID: 23950570
    19. In most cases (90 %), inherited nephrogenic diabetes insipidus (NDI) is an X-linked disease, caused by mutations in the AVPR2 gene. * In rare occasions (10 %), it is caused by mutations in the AQP2 gene. PMID: 25902753
    20. Partial congenital nephrogenic diabetes insipidus in the Swedish family is caused by an AQP2 variation that seems to disable the encoded AQP2-R254W protein to reach the subapical vesicle population as well as impairing its phosphorylation at S256 PMID: 26714855
    21. Taken together these results provide a possible molecular mechanism explaining the increased AQP2 membrane expression under RGZ treatment: in renal cells RGZ elicits Ca(2+) transients facilitating AQP2 exposure at the apical plasma membrane PMID: 25662477
    22. U-AQP2/P-AVP is a novel predictor of response to TLV in patients with decompensated HF. AQP-defined responders may have a better prognosis on TLV treatment PMID: 24954239
    23. In response to hypertonic saline, urinary AQP2 increased more in chronic kidney disease patients compared to controls. PMID: 24970686
    24. results further support the view that urinary calcium can modulate the vasopressin-dependent urine concentration through a down-regulation of AQP2 expression/trafficking PMID: 24885203
    25. AQP2 is subjected to S-glutathionylation, which is modulated by reactive oxygen species production. PMID: 25112872
    26. The genetic polymorphisms in OCT2, AQP2, AQP9 and TMEM205 may contribute to chemotherapy response in lung cancer patients. PMID: 24643204
    27. These observations provide a framework for understanding why mutations in AQP2 cause nephrogenic diabetes insipidus. PMID: 24733887
    28. AQP2 function and thus urine concentration is dependent on a variety of cell signalling mechanisms, posttranslational modification and interplay between AQP2 and its lipid environment--{REVIEW} PMID: 23852332
    29. 9 disease-causing mutations of AQP2 were identified in 9 families. 2 missense mutations and 1 deletion mutation showed recessive inheritance, while 1 missense mutation and 5 small deletion mutations in the C-terminus of AQP2 showed dominant inheritance PMID: 23150186
    30. AQP-2 gene mutation (R254Q) was identified in a family with dominant nephrogenic diabetes insipidus. PMID: 23409988
    31. Together, our data reveal that vasopressin induces instead of reduces the phosphorylation of S261 in AQP2-P262L PMID: 22778181
    32. upregulated Aqp5 may contribute to polyuria, possibly by impairing Aqp2 membrane localization, in Dot1l(AC) mice and in patients with diabetic nephropathy. PMID: 23326416
    33. Investigations into primary inherited nephrogenic diabetes insipidus (NDI) have contributed enormously to our understanding of the mechanisms of urinary concentration and identified the vasopressin receptor AVPR2, as well as the water channel aquaporin-2. PMID: 23364801
    34. Of the 15 patients with diabetes insipidus five patients have AQP2 mutations PMID: 22644838
    35. AQP2 is greatly involved in many human abnormal water balance disorders. (Review) PMID: 23078817
    36. The potential functions of AQP2 and AQP5 in the inner ear are in the resorption and secretion of endolymph. (Review) PMID: 22732097
    37. CaR-AQP2 interplay represents an internal renal defense to mitigate the effects of hypercalciuria on the risk of calcium precipitation during antidiuresis PMID: 22403735
    38. AQP2 mediates E(2)-enhanced migration, invasion, and adhesion through alteration of F-actin and annexin-2 expression and reorganization of F-actin, and inhibition of AQP may be a potential method for antitumor therapy. PMID: 21715543
    39. Our findings would support the involvement of vasopressin-AQP2 axis, interacting with the renin-angiotensin system, in the progression of immunoglobulin A nephropathy and candidate AQP2 as a possible novel marker of the disease PMID: 21824900
    40. The marked early rise in expression of AQP2 and AQP3 suggests a role during the process leading to follicular rupture. PMID: 21252246
    41. After sequencing analysis of the coding regions and exon-intron boundaries one single variation, no significant mutation in AQP 2 was found. PMID: 21063116
    42. endosomal trapping of AQP2 in the endolymphatic sac, might be important as a basis of Meniere's disease PMID: 20722976
    43. Elevation of cAMP increased AQP2 protein levels within 30 minutes in human embryonic kidney 293 cells. PMID: 20724536
    44. Report two new AQP2 mutations displaying high levels of functional expression for mutant forms with adequate plasma membrane targeting in oocytes but not in mIMCD-3 cells where they are restrained within internal stores. PMID: 20403973
    45. We conclude that ibuprofen-induced oligo-anuria is not associated with a change in AQP2 activity and that ibuprofen does not affect AQP2 activity during the first month of life in very preterm neonates. PMID: 20390303
    46. AQP2 associates with detergent-resistant membranes early in biosynthetic pathway. Strong cholesterol depletion delays exit of AQP2 from trans-Golgi network. Association with membrane rafts may regulate both AQP2 apical sorting and endocytosis. PMID: 19923410
    47. Misrouting, instead of a lack of function, is a mechanism for the 'loss of function' phenotype in nephrogenic diabetes insipidus PMID: 11929850
    48. changes in urinary excretion of aquaporin-2 indicates circulatory blood volume depletion, and estimates the AVP-dependent recovery of circulatory blood volume during the therapeutic period in patients with diabetic ketoacidosis PMID: 12021537
    49. Two novel aquaporin-2 mutations responsible for congenital nephrogenic diabetes insipidus in Chinese families. PMID: 12050236
    50. Most AQP2 missense mutants in recessive NDI are retained in the endoplasmic reticulum (ER), but AQP2-T125M and AQP2-G175R were reported to be nonfunctional channels unimpaired in their routing to the plasma membrane. PMID: 12191971

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  • 相关疾病：
    Diabetes insipidus, nephrogenic, autosomal (ANDI)
  • 亚细胞定位：
    Apical cell membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein. Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein.
  • 蛋白家族：
    MIP/aquaporin (TC 1.A.8) family
  • 组织特异性：
    Expressed in collecting tubules in kidney medulla (at protein level). Detected in kidney.
  • 数据库链接：

    HGNC: 634

    OMIM: 107777

    KEGG: hsa:359

    STRING: 9606.ENSP00000199280

    UniGene: Hs.130730